摘要
植物抗病基因克隆对抗病育种和抗病机制的研究具有重要意义。对已克隆出的基因研究表明,大多数的抗病基因都具有高度保守的结构域(如NBS,LRR,LZ,STK和TIR等)。根据这些保守区域设计核苷酸引物,通过PCR技术已经获得很多的RGA,然后以此为探针筛选DNA或cDNA文库,最终可获得抗病基因的候选克隆,这就是新近发展起来的同源序列克隆技术。文章简述了对抗病基因的结构特征,同源序列克隆技术及其应用策略。
Isolation of plant disease resistance gene(R gene) had great significance for plant resistance breeding and the study on disease resistance mechanism. Recent study had showed that resistance genes had common conserved domains, such as nucleotide binding site (NBS), leucine-rich repeats (LRR), leucine zipper(LZ), serine-threonine kinase(STK)and toll-interleukin-1 receptor (TIR). Primers designed based on the conserved domains, many resistance gene analogs (RGA) had been amplified by PCR. Then the RGAs were used as probes to check up the DNA library or cDNA library, and the candidate clones of resistance gene were eventually obtained. This paper reviewed the structure characters of R genes, the application and strategy of homology-based candidate gene method.
出处
《东北农业大学学报》
CAS
CSCD
2004年第4期500-504,共5页
Journal of Northeast Agricultural University
关键词
抗病基因
克隆
类似序列
resistance gene
cloning
resistance gene analogs(RGA)
