摘要
用1～2mmol/L的NaHSO3喷施于野生型水稻Kitaake(WT)、以其为基因受体的转PEPC基因水稻(PC)和转PEPC+PPDK双基因水稻(PC+PK)叶面,叶片的光合速率分别提高了23.0%、28.8%和32.4%,并能持续3d左右.在此条件下,光下叶片中的ATP含量提高.将已知可促进循环光合磷酸化的辅助因子硫酸甲酯吩嗪(PMS)和NaH SO3分别和共同喷洒叶片,次日所有叶片的光合速率均比对照(喷水)提高,共同处理未出现叠加效应,表明NaHSO3的主要作用和PMS促进光合速率的原因类似,都是增加了ATP的供应.与野生型水稻Kitaake(WT)相比,经NaHSO3处理后,转PEPC基因水稻(PC)净光合速率增加的幅度大,转PEPC+PPDK双基因水稻(PC+PK)净光合速率增加的幅度更大,因此认为水稻叶片中增加ATP的供应可明显增强水稻的光合速率,特别是转PEPC或PEPC+PPDK基因水稻.
s:Spraying 1～2 mmol/L solution of NaHSO_3 on leaves of wild type rice Kitaake (WT), PEPC transgenic (PC) and PEPC+PPDK transgenic (PC + PK) germplasm transformed with wild type Kitaake as gene receptor resulted in the enhancement of net photosynthetic rate by 23.0%, 28.8% and 32.4%, respectively, for more than three days. It was also observed that NaHSO_3 application caused increase in ATP content in leaves. Spraying PMS (a cofactor catalyzing cycle photophosphorylation) and NaHSO_3 separately or together on the leaves, increase in photosynthesis had been observed in all the treatments. There was no additive effect when a mixture was applied, suggesting that the mechanism for NaHSO_3 promotion of photosynthesis was similar to PMS, and both of them enhanced the supply of ATP. After spraying solution of NaHSO_3 on leaves, more and most enhancement of net photosynthetic rate were observed in PEPC transgenic (PC) and PEPC + PPDK transgenic (PC+PK) rice, respectively, as compared with wild type rice Kitaake (WT). Therefore the increase of ATP might raise the photosynthetic rates of rice obviously, especially in PEPC or PEPC+PPDK transgenic rice.
出处
《科技通报》
北大核心
2004年第6期523-528,共6页
Bulletin of Science and Technology
基金
国家重点基础研究发展规划项目(G1998010100)
国家自然科学基金(39870533
30270794)
