摘要
目的 探讨神经激肽 A(NKA)在大鼠食管发育中的表达及变化。方法 应用免疫组织化学 PAP法和图象分析术系统研究大鼠胚胎 13d至成年食管中 NKA的表达情况。结果 胚胎 2 1d食管粘膜肌层和环肌层内可观察到 NKA免疫反应 (NKA- IR)阳性膨体纤维 ,出生后 ,随幼鼠的生长发育 ,相继在上皮 ,粘膜下层 ,纵肌层、肌间丛和粘膜下丛出现 NKA- IR表达 ,30 d时已和成年鼠相似 ;定量分析的结果与 NKA- IR在食管各层的变化一致。结论 NKA在食管中的发生发育主要在临出生前至生后 4周内 ;
Objective To explore the expression and change of neurokinin A (NKA) in rat esophagus during development. Methods PAP immunocytochemistry method and image analysis were used to detect systematically the expression of NKA in rat esophagus from the 13th day of embryo to adult stage. Results In rat esophagus, the positive varicosities and fibres could be observed in the circular muscle layer and the muscularis mucosae layer at the 21st day before birth. With the development of rat digestive tract, the neurokinin A-immunoreactivity(NKA-IR) positive nerves could be observed gradually in the epithelium, submucosa, longitudinal muscle layer, myenteric plexus and submucosal plexus, while mature nerve fibres could be seen at one month after birth. The results of quantitative analysis are in accord with the developmental change of NKA-IR in esophagus. Conclusion The ontogeny and development of NKA in rat esophagus proceeds from the last day before birth to 4 weeks after birth. NKA may play an important role in the development of rat esophagus.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2004年第5期619-622,共4页
Journal of Sichuan University(Medical Sciences)
