摘要
目的 研究蝙蝠葛酚性碱对大鼠局灶性脑缺血 再灌注继发炎性损伤的保护作用及其机制。方法 用线栓法制作大鼠右侧大脑中动脉栓塞模型 ,缺血 2h后 ,将线抽出实行再灌注 ,观察蝙蝠葛酚性碱对粘附分子 (ICAM 1)表达、白细胞的粘附与浸润、髓过氧化物酶 (MPO)活性和一氧化氮 (NO)含量的影响。结果 蝙蝠葛酚性碱可明显抑制ICAM 1的表达 ,减轻白细胞的粘附与浸润 ,降低缺血侧大脑皮层和海马组织中的MPO活性和NO含量。结论 蝙蝠葛酚性碱对大鼠局灶性脑缺血 再灌注后的炎性损伤有明显保护作用 ,其机制可能与抑制ICAM 1表达 ,减轻白细胞的粘附与浸润 。
Aim To study the protective effects of phenolic alkaloids from Menispermum dauricum (PAMd) on inflammatory injury following focal cerebral ischemia-reperfusion in rats. Methods The right middle cerebral artery of the rat was occluded by inserting a nylon suture through the internal carotid artery for 2 h, followed by reperfusion by withdrawing the suture. The expression of intercellular adhesion molecule-1 (ICAM-1) was observed by immunohistochemistry staining. The adhesiveness and infiltration of leucocytes were observed by HE staining. The activity of myeloperoxidase (MPO) and the content of nitric oxide (NO) in the cortex and hippocampus were measured. Results PAMd was shown to markedly inhibit ICAM-I expression, alleviate the adhesiveness and infiltration of leucocytes, and decrease the MPO activity and the NO content in ischemic cortex and hippocampus. Conclusion PAMd has protective effects on inflammatory injury following focal cerebral ischemia-reperfusion by inhibiting ICAM-I expression, alleviating the adhesiveness and infiltration of leucocytes and decreasing the generation of NO.
出处
《药学学报》
CAS
CSCD
北大核心
2004年第8期661-665,共5页
Acta Pharmaceutica Sinica
关键词
蝙蝠葛酚性碱
大鼠
局灶性脑缺血
再灌注损伤
炎性损伤
保护作用
phenolic alkaloids from Menispermum dauricum
focal cerebral ischemia-reperfusion
intercellular adhesion molecule-1
myeloperoxidase
nitric oxide
