摘要
目的 观察稳恒磁场对体外培养成年大鼠胚胎脊髓神经细胞生长发育的影响。方法 采用绘制细胞生长曲线的四氮甲基唑蓝 (MTT)法检测和脂质过氧化物的方法 ,了解不同强度的稳恒磁场对体外培养成年大鼠胚胎脊髓神经细胞生长的影响。在相差显微镜下观察细胞生长发育情况 ,并测定其细胞内琥珀酸脱氢酶的活性。结果 1～10mT的稳恒磁场对大鼠胚胎脊髓神经细胞无抑制生长作用 ,当磁场的强度达到 5 0～ 2 0 0mT时其作用才表现出来 ,随着强度的加大 ,其抑制作用增强。结论 一定强度的稳恒磁场对成年大鼠胚胎脊髓神经细胞具有抑制作用 ,且存在强度效应关系。
Objective To observe the effect of static magnetic field(SMF) on the spinal cord neurons in Vitro .Methods Spinal cord neurons were treated with different intension of static magnetic field and the growth of the cells was analyzed by plotting cell growth curve and MTT assay.The growth condition of cell was observed with phase-contract microscope,and intracelluer dehydrase activity was detected.Results 1-10 mT of static magnetic field had no inhibitory effect on the spinal cord neurons.The effect developed when the intension was up to 50-200 mT,and the inhibitory effect of magnetic field became more significant with the increase of the intension.Conclusion Hint to certain strength of static magnetic field had inhibitory effect on spinal cord neurons in vitro ,there was dose-effect relationship intension.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第7期821-822,共2页
Chinese Journal of Public Health
基金
江苏省教育厅自然科学基金资助项目 (0 1KJD31 0 0 0 6)
关键词
稳恒磁场
体外培养
脊髓神经细胞
MTT法
脂质过氧化物
static magnetic field
culture in vitro
spinal cord neurons
MTT assay
lipid peroxidation
