摘要
目的 观察实验性Ⅰ型糖尿病恒河猴的组织病理学变化。方法 7只恒河猴分别静脉注射不同剂量的链脲佐菌素 (streptozotocin ,STZ) ,建立STZ性Ⅰ型糖尿病恒河猴模型 ,观察其心脏、肾脏、胰脏、脾脏等组织病理学变化。结果 动物胰岛数量明显减少 ,分布稀疏 ,残存胰岛萎缩 ,胰岛大小不等 ,成纤维细胞增生。肾小球增大 ,毛细血管壁增厚、僵硬 ,肾小管内膜细胞玻璃样变性 ,肾小球球囊内皮细胞增生。心肌变性、坏死、淤血 ,心肌细胞肥大 ,中、小血管壁增厚 ,血管壁纤维组织增加 ,冠状动脉内膜局部增厚 ,内皮细胞增生。脾脏小动脉硬化。结论 通过对STZ诱发的Ⅰ型糖尿病模型胰岛、肾脏和心脏等结构病理观察说明 ,该动物模型可用于糖尿病组织病理研究和药物疗效的评价。
Objective To observe histopathological change of experimental type Ⅰ diabetic rhesus monkeys. Methods Type Ⅰ diabetic animal models were established in 7 rhesus monkeys by injection intravenously of different doses of streptozotocin(STZ) to observe histopathological variety of their heart,kidney, pancreas and spleen. Results The quantity of pancreatic island decreased obviously, the remainders appeared atrophy and fibroblastic proliferation. In kidney, there were enlarged glomeruli, thickening and rigidity of capillary wall, hycaline degeneration of renal tubular intima cells, hyperplastic endothelia cells of glomerular capsules. Degeneration, necrosis, congestion and hypertrophy were found in myocardium. Thickening of medium-small vessel and hyperplasia of fibrasis tissue were a prominent change in blood vessel wall. Hyperplasis endothelium cells and thickening of endometrium of coronary artery were observed in blood vessel wall. Small arteria in spleen was sclerosed. Conclusion This study shows that the STZ-induced type Ⅰ diabetic animal model is a good model for histopathological study and effective evaluation of drugs.
出处
《中国实验动物学报》
CAS
CSCD
2004年第3期174-176,F003,i003,共5页
Acta Laboratorium Animalis Scientia Sinica
基金
云南省自然科学基金资助 (编号 :1999C0 0 97M)
