摘要
目的 观察重组人血小板源生长因子 (rhPDGF)促进糖尿病大鼠全层皮肤缺损创面修复可能涉及的信号通路。 方法 2 6只糖尿病大鼠背部各制备 4个全层皮肤缺损创面 ,创面随机分成A和B两组 ,A组对照组 ,B组为rhPDGF(7.0 μg/cm2 )治疗组。观察伤后 3,7,14d创面肉芽形成、胶原沉积、再上皮化速率以及炎症细胞浸润情况 ,并采用免疫荧光技术观察创周和创基修复细胞内细胞外信号调节激酶 1/ 2 (extracellularsignal-regulatedkinase 1/ 2 ,ERK1/ 2 )的表达 ,用免疫组织化学方法检测原癌基因c -fos、增殖细胞核抗原 (proliferationcellnuclearantigen ,PC NA)、黏着斑激酶 (focaladhesionkinase ,FAK)的变化情况。 结果 组织学检查显示 ,rhPDGF治疗的创面大量的炎症细胞浸润 ,毛细血管胚芽与成纤维细胞数量显著多于对照组 ,胶原沉积明显 ,肉芽组织生长活跃 ,创面收缩显著。免疫学研究显示 ,伤后 3d ,rhPDGF治疗组ERK1/ 2和c -fos的表达明显增强 ,7d时 ,ERK1/ 2和c -fos进一步增强 ,14d后开始减弱。rhPDGF治疗组各时相点修复细胞PCNA和FAK的表达明显强于对照组。 结论 ERK1/ 2信号通路在rhPDGF促糖尿病大鼠创面愈合中起一定作用 ,并在细胞增殖与迁移中充当重要角色。
Objective To investigate the possible signal transduction pathway during the course of recombinant human platelet derived growth factor (rhPDGF) promoting cutaneous incisional wound healing in diabetic rats. Methods Four full thickness skin wounds were incised in the back of 26 Wistar rats with diabetes mellitus that were randomly divided into two groups, Group A (diabetic rats control) and Group B (treated with rhPDGF at 7.0 μg/cm2 wound). The granulation, collagen sedimentation, the reepithelialization rate of rhPDGF as well as the inflammatory cell filtration were observed 3,7 and 14 days after wound. Immunofluorescence staining was used to observe the expression of ERKs either around the wound and in the wound center and immunohistochemical method applied to observe the changes of c-fos, proliferation cell nuclear antigen (PCNA) and focal adhesion kinase (FAK). Results The histological investigation showed a lot of granule tissues in the bed of wound, a large of inflammatory cells infiltration and collagen deposition, active growth of granule tissues and significant wound contraction. The number of blood capillary buds and fibroblasts in the Group B were more than that in the Group A. The immunohistochemistry showed that the expressions of ERK1 and c-fos increased significantly three days, strengthened seven days and went weak 14 days after rhPDGF application. In the Group B, at every time point, the expressions of PCNA and FAK were more significant than those in the Group A. Conclusions ERK1/2 signal pathway exerts function in rhPDGF accelerating wound healing of rats with diabetes mellitus and plays an important role in proliferation and migration of reparative cells.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2004年第8期452-457,共6页
Chinese Journal of Trauma
基金
国家重点基础研究发展规划资助项目(G1999054204)
国家杰出青年基金资助项目(39525024)
国家自然科学基金重点资助项目(30230370)
国家自然科学基金面上项目(30170966)
博士后基金资助项目(中博基[2001]14)
