摘要
目的:建立高效液相色谱法测定地巴唑片的含量及其有关物质。方法:采用Shimadzu C18色谱柱(4.6 mm×150 mm,5μm);以甲醇-水-三乙胺-冰醋酸(60:40:0.5:0.5)为流动相;流速1.0 mL·min-1;检测波长272 nm。结果:地巴唑的线性范围为16-48/μg·mL-1,r=0.9999;平均回收率为99.83%,RSD为0.62%;最低检测限为1.6 ng;最低定量限为5.2 ng。结论:本法简便、快速、准确,适用于地巴唑片的含量与有关物质测定。
Objective:To establish HPLC method for bendazol tablets and its related substances. Methods:The separation was performed on a Shimadzu - Clg column (4. 6 mm ×150 mm,5 μm). The mobile phase was composed of methanol - water - triethylamine - acetic acid glacial(60: 40:0. 5:0. 5 ). The flow rate was 1. 0 mL·min-1. The detection wavelength was 272 nm. Results: There is a good linear relationship within the range of 16 -48 μg·mL-1 (r =0. 9999). The average recovery was 99. 83% (RSD =0. 62% ). The LOD was 1. 6 ng. The LOQ was 5. 2·ng. Conclusions: The method is simple, rapid, accurate and is suitable for the determination of bendazol tablets and its related substances.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第4期430-431,共2页
Chinese Journal of Pharmaceutical Analysis
