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提高萨福克羊超数排卵的效果研究 被引量:5

Studies on increasing superovulation in Suffolk sheep
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摘要 对 5 4只 (次 )萨福克羊采用CIDR +FSH +PG法进行超数排卵 (以下简称超排 ) ,发情配种后第 6天从子宫角采胚 ,只均获胚数和只均获可用胚数分别为 (6 89± 2 6 2 )枚 (372 / 5 4 )和 (6 0 7± 2 2 8)枚 (32 8/ 5 4 )。并对不同国家产FSH ,注射LRH -A3 +P4,重复超排 ,左、右侧卵巢等影响萨福克羊超排效果的因素进行研究 ,结果表明 ,以加拿大和日本FSH超排效果最好 ,只均获可用胚分别为 (9 2 0± 1 6 4 )枚 (n =10 )和 (7 75± 1 6 7)枚 (n =16 ) ;注射LRH -A3 +P4组只均获可用胚(7 2 5± 1 18)枚 (n =16 ) ,极显著高于对照组 (4 11± 1 38)枚 (n =9) ;重复超排 (间隔 12月 )第 2次超排只均获可用胚(9 19± 3 39)枚 (n =16 ) ,明显高于第一次只均获可用胚 (6 0 0± 3 2 7)枚 (n =16 ) ;左右侧卵巢对萨福克羊超排效果无显著差异。 Suffolk sheep were superovulated by the method of CIDR+FSH+PG and then the embryos were collected from the corners of uterus on the 6th day after estrus and matingThe average number of embryos per sheep was 689±262 (372/54),the average number of useful embryos per sheep was 607±228(328/54),The effects on superovulation of Suffolk sheep were studied from the aspects as following:different FSHin different countries,LRH-A3+P4 injected or not ,repeated superovulation and ovary in left or in righeResults showed that the effect of superovulation by native FSH made in Canada and FSHmade in Japan were better than the others,the useful embryos per sheep were 920±164(n=10)and 775±167(n=16) respectively; 725±118(n=16)of useful embryo was obtained in the group theat injected LRH+P4,which is remarkably higher than that of the control group of 411±138(n=9);The number of useful embryo per sheep of the second time was 919±339(n=16)heigher than that of the first time 600±327(n=16)in repeated superovulation group (the interval is 12 months)There was no obviously difference in the number of useful embryos between left ovary and right ovary
出处 《黑龙江畜牧兽医》 CAS 北大核心 2004年第9期13-15,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 国家科技部"十五"重大专项科技攻关课题(2 0 0 2BA5 14A - 1)子课题 河北省科技厅"十五"重大攻关课题(0 12 2 0 4 35 0 )
关键词 萨福克羊 超数排卵 胚胎移植 FSH Suffolk sheep FSH superovulation
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