摘要
目的 研究新型磺酰脲类化合物G 0 0 3的降血糖作用及其降血糖作用机制。方法 ①小鼠灌胃给药 ,葡萄糖氧化酶法测定血糖值。②离体培养大鼠胰岛、脂肪细胞及小鼠肝细胞 ,研究化合物G 0 0 3对胰岛素分泌、葡萄糖转运和氧化利用及糖原合成的影响。结果 化合物G 0 0 3显著降低正常小鼠空腹血糖水平 ,刺激离体胰岛分泌胰岛素 ,显著促进脂肪细胞转运和氧化利用葡萄糖 ,对离体肝细胞的糖原合成无明显影响。结论 新型磺酰脲类化合物G 0 0 3具有显著的降血糖作用 ,其降血糖作用机制与刺激胰岛分泌胰岛素。
Aim To study the hypoglycemic effect and the mechanism of novel sulfonyluric compound G-003. Methods ① After compound G-003 was givenig for 7 days, the levels of fasting plasma glucose of normal mouse was assayed with glucose oxidase kit. ② Rat pancreatic islets, adipocytes and mouse heaptocytes were isolated and cultured. Effects of compound G-003 on insulin release, glucose transport and utilization and glycogen synthesis were studied. Results Compound G-003 markedly decreased the levels of fasting plasma glucose of normal mouse, stimulated isolated pancreatic rat islets to release insulin and increased glucose transport and utilization in isolated rat adipocytes. Compound G-003 has no effect on glycogen synthesis in isolated mouse hepatocytes. Conclusion Compound G-003 has a significantly hypoglycemic effect. The hypoglycemic mechanism of novel compound G-003 may be attributed to stimulate secretion of insulin and increase glucose transport and utilization.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2004年第9期1026-1030,共5页
Chinese Pharmacological Bulletin
