摘要
目的 从临床流行病学的角度 ,对新的人微小病毒B19非结构蛋白DNA巢式聚合酶链式反应 (PCR)检测方法的效度及信度进行评价 ,探索其运用于临床筛查B19病毒感染的可行性。方法 以国内目前常用的结构蛋白DNA巢式PCR检测结果 ,并结合孕妇的典型临床表现作为诊断标准 ,将 30例孕妇分为B19病毒感染组及非感染组 ,对非结构蛋白DNA巢式PCR的检测结果进行效度及信度评价。结果 新的检测方法血清标本的灵敏度可达 10 0 % ,组织标本的特异度及阳性预测值高于血清标本 ,联合试验有助于提高检测的灵敏度或特异度。结论 非结构蛋白DNA巢式PCR检测方法是一种特异、敏感、简便、快速诊断B19病毒感染的新方法。在临床筛查中 ,血清标本的检测优于组织标本。
Objective To explore the sensitivity and specificity of the new nested-polymerase chain reaction (nested-PCR) assay in the detection of human parvovirus B19 nonstructure protein DNA and evaluate validity and reliability of this diagnostic test in clinical epidemiology for further demonstrating applicability of this new assay in screening patients infected with parvovirus B19. Methods In accordance with the routine detection of structure protein DNA and the typical symptom of virus infection, 30 pregnant women were divided into the virus infected groups and non-infected groups. The validity and reliability of this new assay was evaluated in clinical epidimiology. Results The sensitivity of the new assay for serum samples was up to 100 %. The specificity and positive predictive values in tissue specimens were higher than those in serum samples. The combined assay was helpful to increase the sensitivity or specificity. Conclusion The new nested-polymerase chain reaction assay is a sensitive, specific and convenient method in detecting human parvovirus B19 infection. In clinical screening, detecting B19 virus in serum is better than in constitution. Combined test of structure protein and nonstructure protein DNA PCR can be applied as a diagnostic standard in terms of the purposes.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第4期412-415,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
湖北省计划生育委员会资助课题 (No .0 1LG 0 8 17)
