摘要
采用室内饲养试验方法,研究了H2O2对家蝇幼虫体内过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(AsA-POD)活性的影响。结果表明,外源H2O2能不同程度地影响以上3种酶的活性,但变化规律却有明显差异:CAT活性变化的基本趋势是随着龄期的增加而递增,处理组最大值分别是对照组的1.2、1.26、1.47倍,另外在1、2龄幼虫中CAT活性出现了对照比处理高的现象,而3龄幼虫则没有出现。经H2O2处理后的1、3龄幼虫体内的POD出现了2个活性峰,1龄幼虫被处理后,在第1个活性峰出现的时间内对照明显比处理高,且持续时间约为5h,然后酶活性骤然上升,活性高峰值是对照组的6.8倍;2龄幼虫只有1个活性峰,是对照组的3.68倍;3龄幼虫体内的POD也有2个活性峰出现,分别是对照组的4.9、2倍。AsA-POD活性在整个实验过程中没有出现对照高于处理的现象,1、2龄幼虫经过处理后出现了2个活性峰,分别是对照组的1.3、1.3倍和2.08、1.73倍,3龄幼虫则只有1个活性峰,是对照组的1.8倍。
In order to probe the anti-pesticide mechanism in housefly, we f irstly studied the effects of exogenous H2O2 on the activities of peroxidase, ca talase and ascorbic acid peroxidase in the larvae to simulate the stress situati on. The results showed that H2O2 could change the activities of POD, CAT and AsA-POD to some extent. The activity of CAT increased with the increase of instar . When the larvae were exposed to H2O2, the maximum of CAT activity in the treat ed group was 0.544U (CK: 0.7U), 0.7 (CK: 0.556U), 0.739U (CK: 0.508U), which wer e 1.2, 1.26 and 1.47 times of the control group, respectively. The CAT activity of the first and second instar larvae in the control group were higher than that in the treated group in the early stage after being exposed to H2O2, with the m aximal inhibition rate being 15%in both instar larvae, while these results di d not appear in the third instar larvae. The activity of POD decreased with the larvae growing, and after being exposed to H2O2, the POD activity in the first i nstar larvae was significantly lower than that in the treated group which lasted for about 5 hours with the maximal inhibition rate 65%, then, the POD activit y increased quickly and reached its maximal activity unit (68U), being 6.8 times of that of control group. The second larvae reached the peak of 46.66U, being 3 .68 times of that of control group (12.66U). The activity of POD in the third in star larvae also showed two peaks, with the peak value being 6.6U (CK: 1.34U) an d 5.34U(CK: 2.66U), respectively, which was 4.9 and 2 times of that in the contr ol group. The activities of AsA-POD had two peaks in the first and second inst ar larvae. The peak values were 26 U (CK: 20U) and 22.6U(CK: 17.4U) respectively in the first instar larvae, being both 1.3 times of the control group, while, i n the second larvae, which were 25U(CK: 12U) and 26U(CK:15U), 2.08 and1.73 times of the control group respectively. The third instar larvae had only one peak wi th the activity of 12U, which was 1.8 times of the control group.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2004年第5期954-957,共4页
Journal of Agro-Environment Science
基金
天津市自然科学基金资助项目(033604911)
关键词
家蝇
过氧化氢酶
过氧化物酶
抗坏血酸过氧化物酶
抗药性
housefly (Musca Dometica)
catalase
peroxidase
ascorbate acid per oxidase
pesticide resistance
