摘要
采用水浴浸取法提取烟草中的糖,以Zorbax糖分析柱(柱温为30℃)、乙腈∶水(80∶20,V/V)为流动相和蒸发光散射检测器分离测定烟草中果糖、葡萄糖、蔗糖和麦芽糖的含量。4种糖在含量80～1000mg/L范围内与其色谱峰面积之间具有良好的线性关系,检出限分别为2.0mg/L,1.6mg/L,1.6mg/L,2.0mg/L。
The carbohydrates in tobacco were leached by water, and the leaching proteins were deposited by lead acetate. The supernatant was purified by the C18 solid extraction column. The four carbohydrates, which are fructose, glucose, sucrose, and maltose, were separated on Zorbax carbohydrate analyzing column by using acetonitrile-water (80∶20,V/V) as the mobile phase and with the column temperature at (30℃.) The separating matters were detected by vaporized light-scatting detector (ELSD) with the temperature of drift tube at (85℃) and the air flow rate at 2.1 L/min. The proposed HPLC-ELSD method had a good linearity from 80 mg/L to 1 000 mg/L of the carbohydrates in the leaching solution. The detection limits of the four carbohydrates were 2.0 mg/L, 1.6 mg/L, 1.6 mg/L, and 2.0 mg/L, respectively.
出处
《分析科学学报》
CAS
CSCD
北大核心
2004年第5期531-533,共3页
Journal of Analytical Science
基金
国家烟草专卖局科技攻关项目(No.110220101016)
关键词
高效液相色谱法
蒸发光散射检测
糖
烟草
High performance liquid chromatography
Evaporative light-scattering detector (ELSD)
Carbohydrate
Tobacco
