摘要
目的测定中药柴胡(Bupleurun Chinese DC, BCDC)提取物对人肝癌细胞BEL-7402细胞内游离钙离子浓度([Ca2+]i)的影响,以探索柴胡逆转BEL-7402细胞多药耐药的机制.方法以Fura-2/AM为细胞内钙离子的荧光指示剂,用双波长荧光分光光度计分别测定不同条件下的BEL-7402细胞内[Ca2+]i.结果柴胡可使人肝癌细胞BEL-7402细胞内游离钙离子浓度下降(P<0.001).结论柴胡可使人肝癌细胞BEL-7402细胞内游离钙离子浓度下降,为一种钙离子通道阻滞剂(Calcium channel blocker, CCB),提示钙离子通道阻滞作用为柴胡逆转BEL-7402细胞多药耐药的机制之一.
ObjectiveTo measure the effect of the extracts from BCDC on intracellular free calcium of human hepatoma BEL-7402 cells, in order to explore the mechanism of BCDC reversing the multidrug resistance of BEL-7402 cells of human hepatoma.MethodsDouble wavelength spectrofluorometer with Fura-2/AM as the fluorescence indicator for intracellular free calcium(i) was used to measure the changes of i in BEL-7402 cells of human hepatoma affected by BCDC.ResultsBCDC can reduce intracellular free calcium of human hepatoma BEL-7402 cells (P<(0.001)).ConclusionBCDC is one of calcium channel blocker, which indicate that the effect of calcium channel blocker may be one of the mechanism of BCDC reversing the multidrug resistance of BEL-7402 cells of human hepatoma.
出处
《北华大学学报（自然科学版）》
CAS
2004年第4期323-325,共3页
Journal of Beihua University（Natural Science）
基金
吉林省教育厅科研基金项目(吉教合字2000第26号)
