摘要
目的 为探索SARS的治疗药物 ,实验观察合成的 13种小分子药物抑制SARS冠状病毒的效果。 方法 采用微孔塑料板病变法及MTT法 ,通过观察细胞病变 (CPE)及OD值测定结果 ,判定药效。 结果 13种小分子药物中 3、4、7和 8号药物在接种SARS病毒后 2 0h内对SARS冠状病毒有抑制作用 ;9号药物在接种病毒后 3 0h内药效显著 ;接种SARS病毒 3 6h后 ,SARS病毒繁殖迅速 ,48h后全部发生细胞病变 ,微孔板病变法与MTT法结果一致。 结论 13种药物中有 5种药物在2 4h内对SARS冠状病毒有一定的抑制作用 ,3 0h后所有药物细胞孔开始出现病变 ,48h后细胞全部病变、死亡 ,表明合成的 13种小的药物均不能有效地抑制SARS病毒的繁殖。
Objective To explore the drug against SARS coronavirus and study the anti-SARS virus activities of 13 different synthetic small molecular drugs on the cell culture system. Methods Anti-SARS virus activities of drugs were determined based on the data obtained by using micropore plastic plate and MTT thchnique. Results Within 2 hours after inoculating SARS virus,the drugs of No.3?4?7 and 8 had anti-SARS effect.The drug of No.9 had significant effect within 30 hours.SARS virus proliferated quickly 36 hours after inoculation. CPE ocurred to all cells 48 hours after inoculating SARS virus.Results of the two methods were coincident. Conclusion There are 5 of the 13 drugs have anti-SARS effect to some extent within 20 hours after inoculating SARS virus, and CPE ocurred to all cells 30 hours after in oculation. After 48 hours,and cells died.It showed that all the 13 drugs could not inhibit SARS Coronavims effectinely.
出处
《中国热带医学》
CAS
2004年第5期695-696,共2页
China Tropical Medicine
