摘要
目的 分析用血液分析仪作血小板计数时小红细胞对其计数结果的影响。方法 依仪器血小板计数原理 ,将有小红细胞干扰的标本分成 3组 ,A组 :血小板直方图右峰上翘起始处 >2 5fl;B组 :血小板直方图右峰上翘起始处在 2 0～ 2 5fl;C组 :血小板直方图右峰上翘起始处 <2 0fl。用ED TA K2 抗凝的静脉血 ,分别在Cell Dyn 170 0型血液分析仪上作血小板参数分析 (下称仪器法 ) ,并同时用显微镜目测计数法计数其血小板 ,再对各组两法检测结果作配对t检验。结果 用血液分析仪检测 ,A、B、C 3组平均结果分别为 (199±4 3)× 10 9/L、(2 0 1± 75 )× 10 9/L、(2 90± 5 5 )× 10 9/L ,而显微镜目测计数法A、B、C 3组平均结果分别为 (2 0 0± 4 4 )× 10 9/L、(187± 76 )× 10 9/L、(16 9± 5 0 )× 10 9/L ;其中A组两法差异无统计学意义 ,而B、C两组两法检测结果差异有显著性 (P <0 0 0 1)。结论 由小红细胞引起的异常血小板直方图右峰上翘起始处 <2 5fl时 ,对血小板计数结果有明显影响 ,因此必须正确分析血小板直方图和血小板计数结果 ,如有干扰时必须作显微镜计数复检 ,否则会导致错误的结果。
Objective To analyse incorrect results of platelet counting caused by micro erythrocytes in heamatologic analyser. Methods According to the counting principle in heamatologic analyser and the platelet histogram, we divided blood samples into three groups: group A, group B and group C. The uplifted point of the right peak in platelet histogram was beyond 25 fl, between 20 fl and 25 fl, and less than 20 fl in group A, B and C respectively. Anticogluted with EDTA-K 2 these samples were tested in heamatolgic analyser and counted under light microscope. Then the results of these two methods were compared using paired t test. Results The average results of PLT of group A, group B and group C was (199±43)×10 9/L, (201±75)×10 9/L, (290±55)×10 9/L respectively by heamatolgic analyser and (200±44)×10 9/L, (187±76)×10 9/L, (169±50)×10 9/L under light microscope. The difference between groups B and C was statistically significant. Conclusions The effect of micro erythrocytes on the results of PLT is significant when the uplifted point of right peak in platelet histogram is at less than 20 fl.
出处
《安徽医科大学学报》
CAS
2004年第5期384-385,共2页
Acta Universitatis Medicinalis Anhui
