摘要
目的 :降低mRNA差异显示假阳性。方法 :采用反向Northern杂交方法进行筛查差异片段的真实性。结果 :差异片段再扩增后经检测符合杂交条件的有 96条 ,反向杂交后 ,有 10条DNA片段属于真实的差异片段。结论
Objective:To decrease the false postive rate of differential display PCR.Mehtods:Screeing the differential display PCR result by reverse northern blotting met hod.Result:By DDRT PCR we got 96 bands.The reverse northern blot results showed ten of them were real differential display bands.Conclusion:Reverse northern blotting is a effective mathod to decrease false postive rate of DD PCR.
出处
《中国中医基础医学杂志》
CAS
CSCD
2004年第9期29-30,34,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家中医药管理局科研基金 (0 2 0 3JP47)
创新工程基金(CX 0 0 02 )项目
博士后基金(0 3 3 2 2 7)
关键词
反向Northern
冠心病血瘀证
差异片段
reverse northern blotting
Blood Stasis Syndrome in Coronary Heart Disease
differential display PCR
