摘要
目的 研究白藜芦醇诱导胃癌细胞脱落凋亡的作用。方法 采用细胞形态学观察、Annxin-V和PI细胞染色和流式细胞仪检测,观察白藜芦醇(0.1、0.2、0.5 mmol/L)对脱落培养胃癌细胞B(3C823、SGC7901和HGC27的生长状态、细胞周期的影响及诱导脱落凋亡的作用。结果 倒置显微镜下发现脱落培养的胃癌细胞聚集成团生长,0.1 mmol/L白藜芦醇作用细胞后可抑制抗脱落凋亡的胃癌细胞聚集成团;流式细胞仪检测和Annxin-V和PI细胞染色表明0.5 mmol/L白藜芦醇可分别阻滞脱落培养的BG(2823、SGC7901和HGC27细胞于G0/G1、S、G2/M期,并可诱导这3株胃癌细胞发生脱落凋亡,其中自藜芦醇诱导HGC27细胞脱落凋亡比例达19.3％,远高于其他两株胃癌细胞。结论 白藜芦醇可诱导胃癌细胞发生脱落凋亡。
Object To study the inducing of resveratrol on anoikis of gastric cancer cells. Methods Cell morphology observation, Annxin-V and PI staining, and Flow cytometry (FCM) assay were used to observe the inducing effect of resveratrol (0.1, 0. 2, 0. 5 mmol/L) on cell growth, cell cycles and anoikis of the gastric cancer cells (BGC823, SGC7901, and HGC27) cultured in suspension. Results Cluster growth of gastric cancer cells cultured in suspension was observed by invert microscope. Resveratrol (0. 1 mmol/L) could inhibit the gastric cancer cells resistant to anoikis to form cluster by FCM assay and Annxin-V and PI staining. Resveratrol (0. 5 mmol/L) could arrest the suspended gastric cancer cells (BGC823, SGC7901, and HGC27) to G0/G1, S, G2/M phase respectively and induce the gastric cancer cells to anoikis, and the anoikis rate of HGC27 was around 19. 3% , which was higher than that of other two cells. Conclusion Resveratrol can induce gastric cancer cells to anoikis.
出处
《中草药》
CAS
CSCD
北大核心
2004年第10期1138-1140,共3页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金(30170465
30000067)
军队"十五"科研基金重点课题(01Z080)
