摘要
目的 :探讨散发性人结直肠癌发生发展中可能的信号转导通路。方法 :应用 8条信号通路基因芯片筛选结直肠癌组织与正常粘膜组织表达差异基因 ;提取 35例结直肠癌患者配对的癌组织及正常粘膜组织 (阴性切缘组织 ,距肿瘤 10 cm以上 )总 RNA,以 RT- PCR的方法对有差异的基因进行表达差异比较。结果 :结直肠癌组织中hsf1、hsp2 7及 inos的表达明显高于正常组织。经 35例结直肠癌患者癌组织与正常大肠粘膜组织对比 ,癌组织中hsf1、hsp2 7、inos表达增高 ,其中 hsf1为 86 % (30 / 35 ) ,inos为 6 3% (2 2 / 35 )。结论 :在结直肠癌组织中 hsf1、hsp2 7及 inos基因被激活 。
Objective: To explore the activation of the signal transduction pathways related with the carcinogenesis of sporadic colon cancers. Methods: A gene microarray monitoring activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissue. The differentially expressed genes were further analyzed by RT PCR, using RNA extracted from cancer tissue and matched normal colon mucosa of 35 patients with colorectal cancer. Results: The expression of hsf1, hsf27 and inos was increased in colon cancer compared with normal colon mucosa using PathwayFinder GEArray. The RT PCR results showed that the expression of hsf1 was detected in 86% of patients(30/35)and the expression of inos detected in 63% patients(22/35). Conclusion: Hsf1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2004年第5期390-394,共5页
Journal of Zhejiang University(Medical Sciences)
