摘要
目的 :研究细胞粘附分子Trophinin在着床期小鼠子宫内膜的表达。方法 :收集孕 1～ 6天的小鼠子宫内膜 ,采用RT-PCR技术测定TrophininmRNA的表达水平。 结果 :未孕鼠及孕D1、D2未检测到其表达 ,孕D3、D4、D5大量表达 ,D6仅有少量表达或不表达。结论 :TrophininmRNA在小鼠子宫内膜的表达时间与胚泡着床时间一致 ,提示Trophinin可能参与了胚泡对子宫内膜的粘附。
Objective:To investigate the expression of Trophinin gene in the mouse endometrium during the period of blastocyst implantation.Methods:By reverse transcription-polymerase chain reaction(RT-PCR),Trophinin gene in the mouse endometrium of 1～6 day normal pregnancy was detected.Results:The Trophinin mRNA was not detected in endometrium of nonpregnant and 1～2 day pregnant mouse.But in 3～5 day normal pregnancy it was strongly expressed.The Trophinin gene was scarcely detected in the endometrium of the 6 day pregnancy.Conclusion:Expression time of Trophinin mRNA in mouse endometrium coincides with blastocyst implantation.This suggests Trophinin participate in the adhesion of blastocyst and endometrium.
出处
《重庆医科大学学报》
CAS
CSCD
2004年第5期585-587,共3页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目 (编号 :3 0 2 70 5 10 )
