摘要
〔目的〕建立一种简便、快速、灵敏的测定茶叶中茶多糖含量的方法。〔方法〕按醇析水提法 ,通过正交试验获得茶多糖提取最佳条件 ,苯酚—硫酸法比色测定茶多糖含量。〔结果〕茶多糖吸收波长为 490nm ;线性范围为 8～ 72 μg/ml ;重现性好 ,CV =0 .95 6% (n =6) ;平均回收率为 99.9% ,CV =2 .0 3 7% (n =6) ;不同茶叶样品茶多糖含量在 1.2 92 %～4 5 2 9%之间。〔结论〕该法简便、快速、稳定、灵敏 ,重现性好。
〔Objective〕 To develop a simple,rapid and accurate method for determination of tea-polysaccharide (TPS) in tea.〔Methods〕 TPS was extracted by back-flow with alcohol and water in the samples.The best condition for extracting TPS in tea was determined by orthogonal test.The UV spectrophotometer was used to determine the content of TPS by using phenol-sulfuric acid.〔Results〕 The linear rage was 8-72 mg/ml,repeatability was good (CV=0.956%,n=6) and the average recovery was 99.9%.The content of TPS in different tea samples was 1.292%-4.529%.〔Conclusions〕 This method is simple,rapid and accurate.
出处
《中国卫生检验杂志》
CAS
2004年第4期432-433,共2页
Chinese Journal of Health Laboratory Technology
基金
浙江省医药卫生科学研究基金资助
关键词
茶叶
茶多糖
提取
含量
测定方法
Tea
Polysacchride
Extraction
Determination
Method
