摘要
通过紫外线诱变处理 ,获得了可以在低温下 (1 0℃ )生长的绿色木霉LTR 2的快速生长型突变株LR ,以及对多菌灵具有抗性的突变株LRR。突变株对棉枯萎病菌、棉黄萎病菌、棉立枯病菌的平板拮抗能力一般低于野生型菌株。LR比LTR 2更能适合非根际土壤环境 ,而LRR在健康棉花根际的定殖能力上 ,比LTR 2有明显下降。LR对棉花立枯病基本没有防治效果 ,但对棉花黄萎病和枯萎病的防治效果则高于原始菌株 ;LRR对棉花上述
Mutant LR which is fast growing and grows at low temperature (10℃), and mutant LRR which is resistant to carbendazim (20μg/ml), were obtained from a Trichoderma viride strain LTR-2 by ultraviolet mutation. The two mutants are weaker in inhibiting the pathogens in vitro against cotton pathogens (Fusarium oxysporum f. sp. vasinfectum, Rhizoctonia solani, and Verticillium dahliae) compared to parental strain. LR is more adapted in non-rhizosphere soils, and LRR is less competent in healthy cotton rhizosphere than LTR-2. LR is not effective in controlling cotton damping-off caused by R. solani, but is more effective than LTR-2 in controlling both cotton wilt diseases caused by F. oxysporum f. sp. vasinfectum and V. dahliae, respectively. LRR is similar to LTR-2 in controlling all the three diseases.
出处
《中国生物防治》
CSCD
北大核心
2004年第3期182-186,共5页
Chinese Journal of Biological Control
基金
山东省科技攻关计划 (鲁科计字 (1999)第 2 5 3号 )
关键词
绿色木霉
诱变
紫外线
棉花病害
Trichoderma viride
mutation
ultraviolet
cotton diseases