摘要
本文构建了一个借助微米小球为"手柄",用光镊间接操控生物大分子,进而测定生物大分子之间结合强度的实验 方法。具体实验以抗体A6D蛋白(肌醇磷酸激酶的单克隆抗体)与抗原P14-kinase(肌醇磷脂激酶)为例,对它们之间结合强 度进行了实验研究。该方法可望为生物大分子相互间作用力的定量测量提供直接、有效的研究手段。
A method to measure the adhesion between biomacromolecules was developed, which uses a microbead as 'handle' and indirectly controls the biomacromolecule with optical tweezers. As a model system the adhesion between antibody A6D protein and antigen PI4-kinase was experimentally studied using the method. The result showed that the method could be used effectively for direct measurement of the interaction forces between biomacromolecules quantificationally.
出处
《应用激光》
CSCD
北大核心
2004年第4期233-236,共4页
Applied Laser
基金
国家自然科学基金重大项目(19890380-4)面上项目(10072062)资助课题
