摘要
目的 比较静态和动态旋转系统中构建组织工程化血管模型中血管内皮细胞分泌功能。方法新型可降解材料聚羟基丁酸酯(PHB),用胶原包埋,形成多孔状PHB+胶原管形支架。分离传代、分化人脐静脉内皮细胞,接种于PHB管型支架内腔面,分别在静态、动态旋转系统中培养14 d后,测定血管内皮细胞分泌一氧化氮(NO)、前列环素(PGI2)水平。结果 在动态旋转系统构建组织工程化血管模型中血管内皮细胞分泌NO、PGI2水平显著高于静态系统,在11 d NO分别为(120.52±3.83)μmmol／L、(80.98±5.98)μmmol／L,PGI2分另9为(20.48±1.52)μg／L,(16.59±1.29)μg／L,与静态系统相比,差异有显著性(P<0.05)。结论胶原包埋PHB支架有利于细胞的黏附和生长,可作为组织工程化血管的支架材料。在动态旋转系统构建组织工程化血管模型中血管内皮细胞,具有与正常血管类似的"生理功能"。
Objective To compare the function of the vascular endothelial cell seeded into the surface tissueengineering blood vessel scaffold material in static culture vs rotary cell culture system. Methods The polyhydroxybutyrate (PHB) scaffolds were fashioned into tubular conduits and collagen with reinforcement by PHB. Endothelial cells seeded into scaffold material were isolated from human umbilical vein by enzyme digestion methods and subcultured and purified. After the endothelial cells were cultured in static and rotary cell culture system for 14 days, the levels of nitric oxide (NO) and 6-k-PGFla were measured. Results The secretion levels of NO and PGI2 in vascular endothelial cells of tissue engineering blood conduits in the 'rotary cell culture system' were significantly higher than in the static cell culture system. In rotary cell culture system and static cell culture system, the vascular endothelial cell secreted NO (120.52±3.83) μmmol/L and (80.98±5.98) μmmol/L,PGI2 (20.48±1.52) μg/L and (16.59±1.29) μg/L respectively at 11th day (allP<0.05).Conclusion PHB biodegradable material coated with collagen was benefit for the aderence and growth of the cells. Itmay be an ideal scaffold material. In comparison with the static controls, the 'rotary cell culture system' conduits were similar to that of the natural blood vessels.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第9期1091-1092,i003,共3页
Chinese Journal of Experimental Surgery
