摘要
目的 对前列治胶囊中余甘子、姜黄、赤芍进行薄层色谱定性鉴别,并测定姜黄素的含量。方法 采用薄层色谱法对上述3味药材进行鉴别,展开剂分别为氯仿-醋酸乙酯-甲酸(6:4:1)、正丁醇-浓氨-乙醇(30:3:1)、氯仿-甲醇-水(13:7:2)的下层液;采用反相高效液相色谱法测定姜黄素的舍量,LUNA C18色谱柱(5μm,250mm×4.6 mm),以四氢呋喃-5％醋酸溶液(42:58)为流动相,流速为1.0 mL·min-1,柱温35℃,检测波长428 nm。结果 HPLC测定的线性范围为0.008-0.048μg,相关系数r=0.999 9;胶囊中姜黄素的平均回收率为98.62％,n=6;精密度(RSD<2％)良好。结论 该法操作简便、快速、准确,适用于前列治胶袭中主要药对的定性鉴别和姜黄素的含量测定。
OBJECTIVE To identify fructus phyllanthi, rhizoma curcumae longae, and radix paeoniae rubra, and to determine the content of curcumin in Qianliezhi capsule. METHODS TLC and HPLC methods were used. The samples were separated on LUNA C18 colume. The mobile phase was tetrahydrofuran-5 % aectic acid (42 : 58), at a flow rate of 1. 0 mL·min-1 with the colume temperature 35℃. The detection wavelength was 428 nm. RESULTS The linear calibration curves were obtained over the range of 0. 008 - 0. 048μg (r=0. 999 9). The average recovery of piperine was 98. 62% (n = 6) in the capsule. The precision of the method was good (RSD<2%) . CONCLUSIONS The method is quick, accurate and is suitable for detecting the content of curcumin in Qianliezhi capsule.
出处
《中南药学》
CAS
2004年第5期289-291,共3页
Central South Pharmacy
