摘要
目的:检测幽门螺杆菌(HP)感染患者血清中细胞毒相关蛋白CagA和空泡细胞毒素VacA抗体,观察不同菌株对胃黏膜组织学改变的影响。方法:利用免疫印迹法检测139例HP感染的胃十二指肠疾病患者血清中CagA和VacA抗体,这些患者胃黏膜病理变化均经胃镜证实。观察不同毒力的HP菌株对胃黏膜病理学改变的影响。结果:十二指肠球部溃疡及慢性萎缩性胃炎患者血清中CagA及VacA抗体的检出率明显高于慢性浅表性胃炎的患者,且CagA抗体阳性组患者胃黏膜炎症活动及萎缩的程度较CagA抗体阴性组差异有显著性,而在肠上皮化生方面差异则无显著性。结论:具有CagA及VacA基因的幽门螺杆菌可能在诱导胃黏膜炎症及萎缩方面起了较为重要的作用。
Objective: To differentiate cytotoxin-associated geneA(CagA) and vacuolating cytotoxin gengA(VacA) in people infected with Helicobacter pylori and observe their different virulence in gastric mucosa. Methods: Antibodies, Anti-CagA and Anti-VacA, were tested with immunoblot in 139 patients, whose gastric mucosa pathological changes had been confirmed by gastroendoscopy. Pathological changes were observed and analysed through different strains. Results: The positive rates of Anti-CagA and Anti-VacA antibodies were significantly higher in peptic ulcer and chronic atrophic gastritis. Infection of CagA+ and VacA+ strain was associated with acute inflammation and atrophy of gastric mucosa. But the association with intestinal metaplasis was not obvious. Conclusion: Helicobacter pylori with CagA and VacA gene plays an important role in gastric mucosa inflammation and atroply.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第6期624-625,共2页
Journal of Nanjing Medical University(Natural Sciences)
关键词
幽门螺杆菌
胃黏膜
病理学
诊断
Helicobacter pylori
gastric mucosa
pathology
diagnosis
