摘要
用湖北天门小麦病田土壤中分离的燕麦孢囊线虫(Heterodera avenae)接种,并种植小麦,待小麦收割后,于不同时间内分离孢囊,在不同条件下进行孵化试验。结果表明,在室温下,旬平均9～12℃孵出的幼虫总数多。在15±1℃恒温下,孢囊孵出的幼虫显著高于20℃、10℃、5℃的处理。黄棕壤土浸液、小麦根汁5倍液有抑制孢囊孵化的作用,小麦根汁10倍液对孢囊孵化无显著影响。小麦根汁20倍液对孢囊孵化有利。室外自然条件下。6～10月线虫的孵化不发生。晾干的孢囊在冰箱冷冻室内存放16个月,不影响其孵化率,在7℃下存放16个月,失去活力的卵则较多。病土晾干在室温下存放16个月(过二个夏),孢囊孵出的幼虫数则显著低于存放4个月(过一个夏)的处理。
Wheat was planted after inoculating juveniles of Heterod era acenae collected from Tianmen, Hubei province. Cysts were extracted from the inoculation soils at different time after harvesting for hatching test. The results showed that an average room temperature approperate for juvenile emergence was 9～12℃ on a ten-day period. The number of juveniles emerged at the constant temperature of 15℃. Five-time wheat root juice and the lixiviation liquid of loess soils inhabited the emergence of juveniles, and ten-time and twenty-time root juice did not have significant effects of juvenile emergence. Neither did wheat root exutives suppress the emergence of juvenils. Juveniles failed to emerge outdoor from June to October. The emergence efficiency of nematodes was high when cysts were stored in freezer for 16 months, while many eggs of cysts lost their viability when stored at 7℃ for 16 months. It was safe to store cysts in dry soil at room temperature for as long as four months but would have trouble if stored longer than 16 months.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1993年第6期561-565,共5页
Journal of Huazhong Agricultural University
