摘要
目的 系统解剖与测量小鼠颈动脉系统 ,为小鼠局灶性脑缺血模型的制作提供参数。方法 在手术显微镜下 ,解剖小鼠颈动脉系后 ,用油标卡尺测定从颈总动脉分叉至各部的长度 ,结果以均数表示。并对尼龙单丝线末端的处理进行了较详细的研究。结果 小鼠颈总动脉分叉至大脑前中动脉分叉处的长度较恒定 ,具体为 (8 2 9± 0 5 4 )mm。尼龙单丝线末端经高温处理后 ,缩短的距离不同 ,所形成的球径不同 ,以缩短 1mm效果较好。结论 小鼠局灶性脑缺血模型制作时 ,从颈总动脉分叉至大脑前中动脉分叉处插线的深度不能超过 8 2 9mm。
Objective To dissect and measure carotid artery system and offer some parameter for model of focal cerebral ischemia in mice. Methods The carotid artery system was dissected and the length of every segment from the crotch common carotid artery to every portion were measured with slide gaud under operating microscope in 15 Kunming mice. The results were expressed by mean±standard deviation. The infarct volume was measured by both in vivo MRI and postmortem TTC staining technique. The treatment procedure of distal end of nylon monofilament line was investigated particularly. Results The length from the crotch of common carotid to the crotch anterior and middle cerebral artery was stable, its mean was (8.29±0.54) mm. After the distal end of nylon monofilament was treated with hyperthermy,the shortening distance difference, the diameter of the ball difference, and shortening 1 mm was better. Conclusion When the model of focal cerebral ischemia is fabricated in mice, the length of line insert from the crotch of the common carotid artery; to crotch of anterior and middle artery must keep about 8.29 mm.
出处
《解剖学研究》
CAS
2004年第3期171-173,225,i001,共5页
Anatomy Research
基金
湖北省科技厅科研基金 (WZ15 3 9)
