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IGF2基因对鸡生长及屠体性状的影响及印记状况的研究 被引量:9

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摘要 以明星肉鸡和丝毛乌骨鸡杂交产生的F2代鸡群为实验材料,IGF2基因作为控制鸡生长、屠体性状主基因的候选基因,通过PCR-SSCP方法对IGF2基因多态性进行检测.该基因外显子2中有一个单核苷酸多态性位点,即71位碱基由C突变为G.对F2鸡群个体进行了基因型鉴定,经方差分析表明:该基因对部分生长、屠体性状有显著性影响(如胸角宽、腺胃重、半净膛重等).研究表明该基因可能调控鸡生长、屠体性状的表达,或与控制生长、屠体性状的主基因连锁.进一步分析发现,正反交结果不一致,即杂合子AB(父本等位基因在前)出生重、胸肌重较杂合子BA高,而腹脂重后者较前者高.另选丝毛乌骨鸡家系做IGF2印记状况检测,杂合子与纯合子进行正反交,然后检测后代基因型并选取杂合子,利用RT-PCR-SSCP检测表达情况,结果表明,鸡的IGF2基因不受印记控制.交互效应可能是由于其他遗传因素作用的结果.
出处 《中国科学(C辑)》 CSCD 北大核心 2004年第5期429-435,共7页 Science in China(Series C)
基金 国家重点基础研究发展规划项目(批准号G20000161)北京市自然科学基金重点项目(批准号:5011002)资助
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