摘要
目的 :探讨CTLA4Ig修饰的DC对实验动物免疫功能的影响。方法 :将经CTLA4Ig基因修饰或未修饰DC腹腔注射C5 7BL 6致敏小鼠 ,以致敏或未致敏C5 7BL 6单个核细胞作为反应细胞 ,以未修饰DC细胞及修饰DC为刺激细胞 ,共培养6天 ,采用MTT比色法检测细胞增殖 ,乳酸脱氢酶法测定细胞毒活性。结果 :CTLA4Ig融合蛋白对未修饰DC致敏或未致敏小鼠的同种细胞刺激的增殖反应有明显的抑制作用。CTLA4Ig修饰DC诱导不同组小鼠淋巴细胞增殖反应均明显降低 ,CTLA4Ig融合蛋白对CTL细胞毒活性有显著抑制作用。CTLA4Ig修饰DC对不同组小鼠CTL细胞毒活性均具有抵抗作用 ,未修饰DC细胞对未致敏小鼠以及未修饰DC对致敏小鼠CTL细胞毒活性敏感。结论
Objective:To study the influence of dendritic cells modified by CTLA4Ig on immunological f unction of experimental animals.Methods:The unmodified or modified dendritic cells were injected intraperitoneally into C57BL/6 mice to sensitize them.Peripheral blood mononuclear cells(PBMC) of sensi tized or unsensitized C57BL/6 mice as reaction cells,dendritic cells modified or unmodified by CTLA4Ig as stimulation cells.These two type cells were co-cultur ed for 6 days.MTT assay was used to detect lymphocyte proliferation.Lactate dehy drogenase release method was used to examine the cytotoxic activity.Results:The result showed that CTLA4Ig fusion protein could obviously inhibite lymphocyt e proliferation response of unsensitized or sensitized C57BL/6 mice.Lymphocyte p roliferation responses of different group mice induced by CTLA4Ig-modified de ndritic cells were significant decreased.CTLA4Ig fusion protein could obviously inhibit the cytotoxic activity of cytotoxic T lymphocyte.Modified dendritic cell s could resist the cytotoxic activity of cytotoxic T lymphocytes of different gr oup mice.Unmodified dendritic cells were susceptible to cytotoxic T lymphocytes of mice unsensitized or sensitized by unmodified dendritic cells.Conclusion:Dendritic cells stably expressing CTLA4Ig fusion protein can obviously decrease lymphocyte proliferation response of allogenic mice and reduce the cytotoxic act ivity. [
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2004年第11期765-767,共3页
Chinese Journal of Immunology
基金
国家重点基础研究项目发展规划 (即国家 973计划 )资助项目 (No CB5 10 0 0 8)
国家自然科学基金重点资助项目 (3 983 0 3 40 )
