摘要
目的通过比较血清神经组织蛋白S100(S-100B蛋白)和神经元特异性烯醇化酶(NSE)变化来观察甲基强的松龙对大鼠颅脑损伤的治疗作用。方法将72只SD大鼠随机分为3组,正常组8只,对照组和治疗组大鼠各32只,分为伤后1,6,12,24h4个小组,每小组8只,采用Feenery法造成鼠脑挫裂伤模型后,用酶联免疫检测技术定量检测伤后不同时相点血清S-100B蛋白和NSE水平。结果(1)正常组血清S-100B蛋白为(0.35±0.03)μg/L,NSE水平为(8.35±1.01)μg/L,对照组和治疗组伤后6～24h血清S-100B蛋白和NSE水平均比正常组高(P<0.05);(2)治疗组比对照组血清S-100B蛋白和NSE低(P<0.05)。结论甲基强的松龙对脑损伤有治疗作用。
Objective To observe the role of methylprednisolone in treatment of brain injuries through comparing the changes of serum S 100B protein and neuron specific enolase (NSE) levels. Methods Seventy two rats with brain contusion made by frontoparietal bone windows plasty with extradural hitting were divided into three groups: control group ( n =32), normal group ( n =8) and treatment group ( n =32) that were subdivided into groups 1, 6, 12 and 24 hours after injury. The levels of S 100B protein and NSE were measured at different time points after hitting by ELISA. Results ①The levels of serum S 100B protein and NSE was (0.35±0.03) μg/L and (8.35±1.01) μg/L, respectively in normal group. The levels of serum S 100 B protein and NSE in control group and treatment group (6 24 hours post injury) were higher than those in normal group ( P <0.05); ② The levels of serum S 100B protein and NSE in treatment group were lower than those in control group ( P <0.05). Conclusion Methylprednisolone is effective in treatment of brain injuries.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2004年第11期671-673,共3页
Chinese Journal of Trauma
