摘要
目的:观察外源性神经营养因子-3(neurotrophin-3,NT-3)基因直接转染失神经肌肉的表达及治疗效果。方法:SD大鼠40只,制成腓肠肌失神经支配模型。实验组术后胖肠肌内注射人NT-3质粒10μl(1μg/μl),对照组注射等量生理盐水10μl/d,不同时间点取材采用免疫组化检测蛋白的表达,并进行肌湿重、肌纤维横截面积和运动终板检测。结果:实验组2d后肌细胞出现外源性NT-3免疫组化染色阳性,7 d时达到高峰,14 d和 28 d时有所下降,但与 2 d相比,仍维持在较高水平。而对照组免疫组化染色结果为阴性。实验组与对照组检测指标比较差异有显著性。结论:NT-3基因直接转染可在肌细胞内表达蛋白,为失神经肌肉萎缩的基因治疗提供了初步的理论和实验依据。
Objective:To investigate the expression of NT-3 ge ne in denervated skeletal muscle and its treatment
in vivo. Methods:40 SD rats were made the model of d enervated muscle atrophy by cutting the right sciatic nerves and
randomly divided into two groups:experimental group in which NT-3 genes was injected in the gastrocnemius muscle and
control group in whichthe same amount of saline was injected. At different postoperative interval,the expressions of NT-
3 protein in rat muscle determinated by histochemistry, the muscle wet weighs,the muscle fiber cross-sectional areas
and acetylcholin esterases were detected to evaluate result of treatment. Results:Positive staining was apparent in the
vicinity of the gastronemius muscle 2 days after injection and reached peak 7 days later,but gradually decreased on 14th
and 28th days in experimental group. There was negative staining in control group. Significant difference was found in
other indexes between the two groups. Conclusion:It shown that NT-3 genes could be efficiently expressed by direct in-
jection in rat denervated skeletal in vivo. The results provided elementary theory for the genes treatment of neuotrophic
factors.
出处
《中国误诊学杂志》
CAS
2004年第6期819-821,共3页
Chinese Journal of Misdiagnostics
关键词
神经营养因子3
遗传学
基因疗法
肌萎缩
治疗
转染
肌
代谢
Neurotrophin 3/genetics
Gene therapy
Denervation
Musclar atrophy/therapy
Transfection
Muscles/metabolism
