摘要
目的 对常用的细菌采样方法的采样效率进行对比。方法 分别用棉拭子采样倾注培养法、棉拭子采样直接涂抹接种于营养琼脂法以及 RODAC印压法 3种常用的细菌采样方法 ,对不同菌液浓度制备的实验菌板和保洁后医院环境进行采样、培养、计数 ,比较采样效果。结果 当制备菌板的菌浓度为 10 8～ 10 5CFU/ m l时 ,用倾注法采样 ,菌落数可通过稀释较准确的获得 ,而 RODAC和涂抹法采样则菌落连成片 ,无法计数 ;当制备菌板的菌浓度为 10 4 ～ 10 2 CFU / m l时和保洁后医院环境采样 ,RODAC法对细菌的捕获力优于倾注法和涂抹法 (P <0 .0 5 )。结论 倾注法适用于物体表面细菌量多时采样 ;而细菌量少时 ,RODAC方法优于倾注法和直接涂抹法。
OBJECTIVETo evaluate the three commonly used sampling methods for bacteria. METHODS The numbers of the bacteria sampled by premoistened swabs which were diluted and inoculated into liquid agar or inoculated direct onto nutrimental agar plate or sampled by replication organism detection and counting (RODAC) imprint technique from known concentration surfaces and hospital environmental surfaces after cleaning were compared. RESULTSWhen sampled from the surfaces with bacterial concentration of 108-105 CFU/ml, we could get relatively accurate bacteria countings after diluted for several times by the method of premoistened swab diluted and inoculated into liquid agar, but could′t recognize and count the bacteria colonies sampled by the method of premoistened swab which was inoculated direct onto nutrimental agar plate and RODAC imprint technique; when sampled the surfaces with bacterial concentration of 104-102 CFU/ml and the hospital environmental surfaces after cleaning, the RODAC method was prior to the other two methods (P<0.05). CONCLUSIONSFor samples from surfaces with large number of bacteria method of premoistened swab diluted and inoculated into liquid agar is suited, while for samples from surfaces with relatively less bacteria the RODAC imprint technique is better.
出处
《中华医院感染学杂志》
CAS
CSCD
2004年第1期35-37,共3页
Chinese Journal of Nosocomiology
