摘要
为深入了解果树光化学反应中心光能分配的状况 ,以柑橘为试材 ,采用调制荧光法对叶片光系统在高光强和低光强下的状态转换进行了研究 .结果表明 ,光系统在 10 0 μmol·m-2 ·s-1的低光强下 ,由于QA 的还原使PQ库处于还原状态 ,导致光能由PSⅡ转向PSⅠ分配 ,光系统处于状态 2 ;在 10 0 0 μmol·m-2·s-1的高光强下 ,PQ库无法得到电子而处于氧化状态 ,导致光能分配由PSⅠ转向PSⅡ ,光系统处于状态1.叶片经磷酸酯酶抑制剂NaF处理后 ,光系统从高光强下状态 2到状态 1的转换受到抑制 .高光强下过多的光能由PSⅠ向PSⅡ分配是导致PSⅡ光破坏的重要原因 .
In order to further understand the light energy distribution in photochemical reaction centers of fruit trees,this paper employed modulated fluorescence technique to study the state transition of photosystem in Citrus unshill leaves under high and low light intensities.The results indicated that under low light intensity of 100 μmol·m -2 ·s -1 ,the PQ pool was in reduction state due to Q A reduction,which made the light energy distribution in photosystem changed from PSⅡ to PSⅠ; while under high light intensity of 1 000 μmol·m -2 ·s -1 ,the PQ pool could not get electron and was in oxidation state,making light energy distribution changed from state 2 to state 1.When the leaves were treated by a phosphatase inhibitor NaF,the state 2 to state 1 transition of photosystem was inhibited under high light intensity,and the excessive light energy distribution from PSⅠ to PSⅡ caused damage to PSⅡ.These findings would be helpful to understand the mechanisms of the photoinhibition of photosynthesis and the photodamage of photosynthetic apparatus of fruit trees.
出处
《应用生态学报》
CAS
CSCD
2004年第11期2087-2090,共4页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目 (3 9970 5 2 5 )
浙江省自然科学基金资助项目 (3 99470 )
关键词
温州蜜柑
光强
叶绿素荧光
状态转换
PQ库
Citrus unshiu, Light intensity, Chlorophyll fluorescence, State transition, PQ pool.
