摘要
目的 :研究新型气体分子硫化氢 (H2 S)对自发性高血压 (SHR)大鼠高血压形成期主动脉几何形态和显微结构的影响并初步探讨其调节主动脉结构重建的作用机制。方法 :4周龄雄性SHR及正常血压 (WKY)大鼠各自随机分为对照组、+NaHS(H2 S供体 )组及 +PPG(H2 S代谢酶抑制剂 )组 ,5周以后 ,应用图像采集与分析系统对Weigert染色的胸主动脉显微形态结构做定量分析 ,应用免疫组织化学的方法检测细胞增殖核抗原 (PCNA)。结果 :9周龄SHR对照组大鼠血压显著高于WKY对照组大鼠 [(184± 12 )mmHg对 (10 8± 2 3)mmHg],而SHR +NaHS组大鼠的血压 [(15 8± 12 )mmHg]显著低于SHR对照组。SHR对照组大鼠胸主动脉的血管内径、外径、中膜面积以及壁厚与内径的比均显著高于WKY对照组 [血管外径 (1999± 4 5 ) μm对 (1790± 96 ) μm ,内径 (175 9± 91) μm对 (16 36± 94 )μm ,中膜面积 (0 .6 0± 0 .0 6 ) μm2 对 (0 .4 8± 0 .0 3) μm2 ,壁厚与内径的比 (0 .0 6 6± 0 .0 0 6 )对 (0 .0 6 0± 0 .0 0 4 ) ],血管平滑肌细胞增殖指数也高于WKY对照组 [(0 .2 4± 0 .0 6 )对 (0 .11± 0 .0 5 ) ]。SHR +NaHS组大鼠的多数血管结构指标低于SHR对照组大鼠 [外径 (186 4± 6 6 ) μm ,内径 (16 34± 6 6 ) μm ,中膜面积(0 .5 3?
Objective: To explore the role and the possible mechanism of hydrogen sulfide (H 2S) in modulating the remodeling of aorta of hypertensive rats. Methods: Twenty-four male WKY rats were randomly divided into WKY control group, WKY+ NaHS (H 2S donor) group and WKY+PPG group. Another twenty-four male SHR rats at the age of 4 weeks were also divided into SHR control group, SHR+NaHS group and SHR+PPG group. SHR control and WKY control rats were injected with water, rats of SHR+NaHS group and WKY+ NaHS group were injected with NaHS, rats of SHR +PPG group and WKY +PPG group were injected with PPG each day. Five weeks later, the blood pressure was determined. The rat aortas were dyed with Weigert’s Method. The morphometric parameters including outer radius, lumen radius and the media area were calculated by Leica workstation. The proliferation of smooth muscle cells was detected by immunohistochemical assay of PCNA. Results: The morphometric parameters of outer radius, lumen radius, medium area, and the ratio of wall thickness to lumen radius in SHR control rats were all higher than those of WKY control rats [outer radius (1 999±45) μm vs (1 790± 96) μm, lumen radius(1 759±91) μm vs (1 636±94) μm, medium area (0.60±0.06) μm 2 vs (0.48± 0.03) μm 2, and the ratio of wall thickness to lumen radius (0.066±0.006) vs (0.060± 0.004)]. Expression of PCNA in aorta of SHR control rats was higher than that of WKY con-trol rats too. Most of the structural remodeling parameters decreased in the rats of SHR+NaHS group [outer radius(1 864±66) μm, lumen radius (1 634±66) μm, medium area (0.53±0.06) μm 2] compared with those in rats of SHR control group. Expression of PCNA in aorta of SHR+NaHS control rats decreased compared with that of SHR control rats at the same time. Conclusion: H 2S is one of the key factors playing important roles in the modulation of the structural remodeling in aorta of SHR rats. Exogenous administration of H 2S may attenuate the development of hypertensive rat aorta remodeling effectively.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2004年第3期234-237,共4页
Journal of Peking University:Health Sciences
基金
教育部教育振兴行动计划特殊专项 ("九八五"工程
8810 5 612 2 0 10 17)
国家重点基础研究发展规划项目基金(G2 0 0 0 0 5 690 5 )资助~~
