摘要
目的 探讨治疗放创性全厚皮肤缺损创面的方法及效果。 方法 贵州小香猪 8只 ,每只背部脊柱两侧均有放创性全层皮肤缺损圆形创面 (Ф 3.6 7cm)各 3个 ,共 4 8个创面。将经处理的人羊膜 (human amniotic mambrane,HAM)分别负载自体骨髓间充质干细胞 (mesenchymal stem cells,MSCs)和表皮细胞 ,移植到其左侧 2 4个创面作为实验组 (A组 ) ;以单纯无种植细胞的 HAM敷盖其右侧前 16个创面 (B组 ) ;以单纯油纱布敷盖其右侧后 8个创面 (C组 )。B、C作为对照组。观察移植后 1～ 3周内各组创面愈合、肉芽组织生长及上皮化等情况 ,并进行创面组织 HE染色及 v WF免疫组织化学检测。用图像分析法测算各组各时间点创面平均面积 (cm2 ) ,并计算其愈合百分率。 结果 C组于伤后2 2～ 2 3天愈合 ,B组于伤后 19～ 2 1天愈合 ;A组于伤后 15～ 17天愈合 ,较 B、C组分别提前 6～ 7天和 5～ 6天 ,愈合质量好。移植 15～ 17天 ,A组与 B、C组创面平均残留面积及愈合面积百分率比较 ,差异有统计学意义 (P<0 .0 1)。 A组创面的新生上皮已完全覆盖整个创面 ,肉芽组织生长旺盛 ,肉芽组织中 v WF、成纤维细胞和毛细血管含量丰富 ,可见胶原沉积 ;B、C组创面仍见许多炎性细胞浸润 ,肉芽组织中 v WF、成纤维细胞和毛细血管含量少 ,?
Objective To investigate the results of human amniotic membrane(HAM) which are loaded with marrow mesenchymal stem cells(MSCs) and epidermis cells in treating full-thickness skin defect combined with radiation injury.Methods Eight minipigs were used in this study. Three round full-thickness wounds(Ф3.67cm), which combined with radiation injury, were created on the dorsum of each side close to the vertebral column in each animal. Among 48 wounds, 24 left side wounds were treated with HAM loaded with MSCs and epidermis cells as experimental group (group A), 16 right side wounds with simple HAM (HAM group, group B) and 8 right side wounds with oil gauze as control (group C). The granulation tissue, reepithelization and wound area were observed after 1,2 and 3 weeks. Immunohistochemistry was performed using vWF as a marker for blood vessels. Image analysis was employed to test new area of wound at different interval time and healing rate of wound.Results The healing time of group A was 6 to 7 days faster than that of group C and 5 to 6 days faster than that of group B. After 15-17 days of graft, there were significant differences in new area of wound and healing rate between group A and groups B,C(P<0.01). New epidermis fully covered whole wound surface in group A, and their granulation tissue, which contained a lot of vWF, fibroblasts, capillaries and collagen, grew well. Many inflammatory cells still were seen in groups B and C, and their contents of vWF, fibroblasts, capillaries and collagen in granulation tissue were smaller than that in group A.Conclusion The graft of HAM loaded with MSCs and epidermis cells played an effective role in promoting healing of wound combined radiation injury with high quality.
出处
《中国修复重建外科杂志》
CAS
CSCD
2004年第6期497-501,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家重点基础研究发展计划 (973)资助项目(G1 9990 542 0 5)
国家自然科学基金资助项目 (30 2 0 0 1 4 2 )~~
关键词
骨髓间充质干细胞
表皮细胞
人羊膜
放创性皮肤损伤
创面愈合
Marrow mesenchymal stem cells Epidermis cells Human amniotic membrane Wound combined with radiation injury Wound healing
