摘要
目的 :探讨中药清肠栓防治溃疡性结肠炎 (UC)作用及其影响细胞间粘附分子 - 1 (ICAM - 1 )的作用机制。方法 :将实验大鼠随机分为空白组、柳氮磺胺吡啶 (SASP)组、模型组、清肠栓高、中、低组共 6组 ,除空白组外 ,其余各组大鼠用兔结肠粘膜蛋白免疫法制作实验UC模型 ,并进一步随机区分预防与治疗 2个实验组 ,而后按预防组第 2～ 2 1天 ,治疗组第 2 2～ 35天分组给药后 ,断头取结肠组织 ,1 0 %福尔马林固定 ,石腊切片 ,免疫组化染色后光镜下视察 ,按统一标准评分 ,Ridit统计分析。结果 :空白组大鼠结肠粘膜固有层及粘膜下血管内皮细胞和巨噬细胞仅表达少量的ICAM - 1蛋白分子 ,造模组大鼠大量表达 ,清肠栓与SASP组较模型组明显减少 (P <0 0 1 )。结论 :中药清肠栓对实验性大鼠UC具有良好的防治作用 ,其机理可能与抗粘附分子有关。
Objective: To study the effect of the prevention of traditional Chinese drug Qingchang plug on ulcerative colitis (UC) and the mechanisms of action on ICAM-1 in cell.Method:The mice in random were divided into six groups,coutrol,SASP,model and three groups of drug.Each group,except control group,was made into animal model of UC and divided into preventive group and therapy group.Subsequenthy,preventive group from 2th to 21th day and therapy group from 22th to 35th day were respectively administered with the drug.After treatment,the observation of colitis tissue obtained from killed mice under microspectro was made.The results were analysed with statistics.Results: The mice of control group expressed less amount of ICAM-1 protein molecular.The cells in animal model group expressed large amount of ICAM-1 protein molecular.Compared with model group,drug groups and SASP were obviously decreased (P<0.01)
出处
《浙江中医学院学报》
2004年第6期44-46,共3页
Journal of Zhejiang College of Traditional Chinese Medicine
