摘要
本实验通过卵巢发育分级的解剖观察、可溶性蛋白质和核酸的定量测定、火箭免疫电泳定量测定卵黄原蛋白及激素处理等方法,研究了早熟素对家蝇(Musca domestica vicina)卵黄发生的影响。试验结果表明用20μg早熟素处理每头刚羽化家蝇时,家蝇卵黄发生处于不完全抑制状态,其卵黄发生过程比对照组“延迟”约12小时。处理后48小时,血淋巴中卵黄原蛋白的滴度为10.5μg/μl,接近对照组,而其卵巢鲜重和发育等级明显低于对照组,这种不完全抑制状态表明卵母细胞对卵黄原蛋白的吸收作用受到抑制。当用高剂量100μg早熟素Ⅱ处理每头刚羽化家蝇时,血淋巴中卵黄原蛋白滴度、卵巢鲜重及其发育均受到明显的抑制,这种抑制效应能自然恢复。当早熟素Ⅱ和保幼激素(JH-Ⅲ)、20-羟基蜕皮酮共同处理时,保幼激素具有明显的去抑制作用,可使血淋巴中卵黄蛋白浓度成倍增加,20-羟基蜕皮酮的去抑制效应不明显。本文还对早熟素作用于双翅目昆虫的方式作了讨论。
Through observations on the rate of ovarian development, quantitative analysis of soluble protein and nucleic acid, rocket immunoelectrophetic measurment of vitellogenin titer in hae-molymph and hormone treatments, the inhibitory effect of precocene II on vitellogenesis in the housefly Musca domestica vicina was investigated. When the newly-emerged femalehouse-flies were topically treated with precocene II in 20 μg/fly, vitellogenesis was temporally inhi-bired and the development was delayed 12 hours than that of the control. At 48 hours after treatment, the titer of vitellogenin in haemolymph was 10.5 μg/μl, nearly the same as the con-trol, but the development of ovary was retarded as compared with the control. This temporal inhibitory effect seemed to indicate that the uptake of vitellogcnin by oocyte was partially in-hibited. When the treatment was in high doage abom 100 μg per fly, the titer of vitellogenin in hemolymph and the weight of overies, toral protein and RNA in ovaries were obviously lo-wer when compared with the control, indicating that the development of the ovary and the syn-thesis of vitellogenin wer inhibited It was observed that the inhibition could be restored natu-rally. When the treatment of precocene II was followed with application of JH III, the inhibi-tion of vitellogenesis was not conspicuous and the liter of vitellogenin in haemolymph was two times that in temporally inhibited flies. When precocene II treatment was followed with topical application of 20-hydroxyecdysone, the vitellogenesis was still inhibited. The mode of action of precocene on ovarian development of dipteran insect is discussed.
出处
《昆虫学报》
CAS
CSCD
北大核心
1993年第2期129-137,共9页
Acta Entomologica Sinica
关键词
家蝇
早熟素Ⅱ
卵黄发生
Musca domestica vicina-precocene II-vitellogenesis-vitellin
