摘要
植物转化是开展基因工程育种和鉴定基因功能的重要途径。本研究的目的在于向大豆受体导入热激转录因子8(heatshockfactor8,hsf8)基因,以加强目的基因hsp70的表达和增加转基因大豆的抗逆性,同时探索提高大豆转化率的途径。本文以大豆子叶节为外植体,通过农杆菌介导法将hsf8基因转入栽培大豆品种科新3号中,获得了13株抗卡那霉素的转化植株。PCR检测表明,其中的9株呈阳性反应,证明hsf8基因已整合到大豆基因组中。本实验获得的实际转化率为2.39%。文中讨论了选择适宜的种子灭菌方法和大豆受体品种对提高转化率的有效作用。
Plant transformation is an important approach in genetic engineering breeding and identification of gene function. Objective of this study is to transfer heat shock factor8 (hsf8) gene into soybean variety and improve expression of target gene hsp70 in order to enhance heat tolerance of soybean. In this experiment, hsf8 was transferred into soybean cultivar Kexin3 through Agrobacterium-mediated method. Thirteen kanamycin-resistant transgenic plants were obtained, and these plants were examined by PCR amplification, and nine of them showed positive signals,indicating that hsf8 gene was integrated into genomes of these nine transgenic plants. The frequency of soybean transformants in this experiment was 2.39%. In addition, the effects of improve the frequence of transformation were discussed, including the suitable sterilization method of the seed and genotype of soybean.
出处
《分子植物育种》
CAS
CSCD
2004年第6期783-787,共5页
Molecular Plant Breeding
基金
国家自然科学基金课题(30270836)
836项目的资助。
关键词
农杆菌介导
热激转录因子8基因
大豆
转基因
种子灭菌法
Soybean (Glycine max), Cotyledonary node, Heat shock factor8(hsf8)gene, Agrobacterium-mediated transformation
