摘要
目的 探讨三磷酸腺苷 (ATP)和神经生长因子 (nervegrowthfactor ,NGF)联合使用时对体外培养的乳鼠脊髓神经元的作用。 方法 利用神经细胞培养技术 ,根据不同干预分为NGF组、ATP组、ATP加NGF组以及单纯对照组。相差倒置显微镜观察细胞生长情况 ,并测量细胞突起的长度 ;用MTT法测定培养细胞的存活率。 结果 实验各组神经元的存活率和轴突的长度均明显优于对照组 ,差异有非常显著性 (P <0 .0 1)。比较MTT值 :ATP组与ATP加NGF组之间差异有非常显著性 (t =4.2 5 ,P <0 .0 1) ;NGF组与ATP加NGF组之间差异有显著性 (t =3 .5 0 3 ,P <0 0 5 )。比较细胞突起长度 :ATP加NGF组分别与ATP组、NGF组之间差异有非常显著性 (P <0 .0 1)。 结论 ATP、NGF对于体外培养的脊髓神经元的存活均有较强的维持作用 ,并能促进轴突生长 ;而两者联合使用作用明显增强。
Objective To explore combined effect of adenosine triphosphate(ATP) and nerve growth factor(NGF) on the cultured neurons of spinal cord in neonatal Wistar rats in vitro . Methods ATP, NGF, ATP+NGF were added into the cultured neurons of spinal cord in the neonatal rats of 1 day old while PBS was added as control. Phase contrast microscope observations, measurement of the length of axon, MTT methods were used to evaluate the survival and activity of the cultured neurons. Results The cell survival rate and length of axon in experimental groups were superior to those in the control group( P <0.01). Significant difference was found between ATP group and ATP+NGF group in MTT( t =4.25, P <0.01); There was significant difference between NGF group and ATP+NGF group in MTT( t =3.503, P <0.05); no difference was found between ATP group and NGF group in MTT( t =2.349, P >0.05). Significant difference was found between ATP+NGF group and ATP group about the length of axon( P <0.01); There was significant difference between ATP+NGF group and NGF group about the length of axon ( P <0.01). Conclusion Both ATP and NGF can enhance neuronal survival and prompt axonal growth in vitro, and combined application of ATP and NGF can produce better results. [Key words] Adenosine tri phosphate; Nerve growth factor; Neurons; Cell culture
出处
《中华显微外科杂志》
CSCD
北大核心
2004年第4期278-280,共3页
Chinese Journal of Microsurgery
