摘要
目的 研究卡介苗(BCG)和尘螨(Dp)对哮喘儿童外周血单个核细胞(PBMC)表达CD19、CD80的影响,探讨CD80在哮喘发病中的作用。方法 收集哮喘儿童15例PBMC,在BCG和Dp刺激后用流式细胞术双标法检测CD19+CD80+细胞百分率结果 BCG组CD19+CD80+细胞降低(27.06±14.40)％,Dp组CD19+CD80+细胞增加(50.29±9.95)％,与对照组(34.83±16.56)％比较有显著差异。结论 CD80在哮喘发病中起重要作用,BCG抑制其表达,Dp促进其表达。 实用儿科临床杂志,2004,19(12)
Objective To study expressions of CD19 and CD80 of peripheral blood mononuclcar cells(PBMCs)stimulated by bacillus calmette guerin (BCG) and dermatophagoldes pteronysslnus(Dp)and to explore the role of CD80 in asthmatic pathogenesis. Method Pereentages of CD19 and CD80 positive cells were counted by two - colored flow cytometry after PBMCs of 15 asthmatic children were stmiulated by BCG and Dp. Result CD19 and CD80 positive cells were decreased in BCG group(27.06 ± 14 .40) %,whereas increased in Dp group(50.29 ± 9.95) % compared with control group(34.83 ±16. 56) % . Conclusions CD80 may play an important role in asthmatic pathogenesis BCG restrains the expression of CD80,whereas Dp induces it.J Appl Clin Pediatr,2004,19(12): 1026 - 1027
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2004年第12期1026-1027,共2页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金资助项目(30271380)
关键词
哮喘
细胞因子
CDRn
卡介苗
尘螨
asthma
cytokine
CD80
bacilltis calmette guerin
dermatophagoldes pteronysslnus
