摘要
采用McAbs免疫酶和PCR技术研究35例淋巴细胞白血病细胞分化起源及IgH和TCR_(γ、δ)基因重排。结果表明:20例表达B细胞表面标记的病例中,15例B-ALL分别起源于B祖、前前B、前B和成熟B细胞,5例B-CLL均起源于成熟B细胞;17例检测到IgH基因重排,8例伴TCR_γ基因重排,2例伴TCR_δ基因缺失。8例T-ALL分别起源于幼稚、普通和成熟胸腺细胞,均检测到TCR_γ基因重排和/或TCR_δ基因重排/缺失。3例T、B-双标记ALL同时发生IgH和TCR_γ基因双重重排。4例AUL中3例发生TCR_γ基因重排。并讨论了细胞分化与基因重排间的相关性及临床意义。
The cellular differentiated origin and the rearrangements of IgH and TCRr.TCRs genes within 35 cases lymphocytic leukimta were studied by irnmunoenzymatic method with McAbs and PCR technique. The results suggested that the 15 cases of B-ALL origined from progenitor B, pre-pre-B,pre-B and mature B cells , respectively and all 8 cases of H-CLL from mature B cells. Out of them. 17 cases showed IgH gene rearrangement, 8 cases had TCRr gene rearrangement and deletion of TCR?gene was detected in 2 cases. 8 cases T-ALL was origined from immature, common and mature thymus cells, respectively and all were demonstrated the rearrangements of TCR. gerie and/or rearrangement or deletion of TCRa gene. The double-rearrangement of IgH and TCRr genes were found in 3 cases of biphenotype ALL and 3/4 cases of AUL showed TCRrgene rearrangement. The relation among cellular differentiated origin and antigen receptor gene rearrangements and its clinical significance were also discussed.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1993年第4期243-246,共4页
Immunological Journal
关键词
白血病
聚合酶链反应
基因重排
Leukemia, Lymphocytic, Differentiation antigen, Polymerase chain reaction, Gene rear- rangement, Immunoglobulin in heavy chain. T cell receptors
