摘要
运用反相高效液相色谱对舒胸片中的有效成分人参皂甙Rg1进行了分离,并对其进行了含量测定,建立了反相HPLC测定舒胸片中人参皂甙Rg1的含量的方法.色谱柱:AlltimaC18(250mm×4.6mm,5μm),流动相:乙腈水(0.05%磷酸)=2575,流速:0.9mL/min,检测波长:203nm,柱温:35℃.平均回收率为96.5%,RSD为1.40%.
In this article, the chemical components of the Shuxiongpian were isolated and the contents of (ginsenoside) Rg1 in the Shuxiongpian were determined by reversed-phase high-performance liquid (chromatography) (RP-HPLC) on Alltima C_(18) column(5μ,250 mm×4.6 mm)with UV detector, at the wave length of 203 nm, at 35 ℃.A mixture of water and phosphoric acid and acetronitrile [CH_3CN:H_2O(0.05% phosphoric acid )=25:75] were used with a flow rate of 0.9 mL·min^(-1). The average recovery and relative standard deviation of this method were 96.5% and 1.40%.
出处
《南昌大学学报（工科版）》
CAS
2004年第4期43-45,共3页
Journal of Nanchang University(Engineering & Technology)
