摘要
目的探讨Schwann细胞与筋膜共同培养的方法 ,为神经缺损修复提供实验基础。方法将Schwann细胞与筋膜共同培养 ,免疫组织化学双染、相差显微镜连续观察及间接免疫荧光染色显示Schwann细胞胶质特异性标记蛋白S 10 0表达和BrdU掺入。结果相差显微镜下可见筋膜周围有大量Schwann细胞 ,且生长迅速 ,胞体呈梭形 ,核为椭圆形 ,胞体两侧有两个细长的突起 ,并呈平行排列 ,少见成纤维细胞。形态学连续观察和S 10 0间接免疫荧光染色都显示BrdU掺入。筋膜BrdU和S 10 0免疫组织化学双染可见筋膜上有大量的BrdU和S 10 0双染阳性的Schwann细胞 ,胞核为深蓝色 ,胞浆红褐色。结论在筋膜上有大量具有增殖活性的Schwann细胞 ,该筋膜可望用于修复神经缺损。
ObjectiveTo explore the method of co-culture of Schwann cell(SCs) with fascia and provide experimental basis for repairing transected nerve.MethodsSCs were co-cultured with fascia.Double staining by anti-BrdU and anti-S-100,S-100 fluorescent staining and anti-BrdU staining were used.ResultsThere were a plenty of SCs around fascia proliferated rapidly and disposed in parallel. SCs could be distinguished from fibroblastic cells by S-100 fluorescent staining and also be staining positive by anti-BrdU antibody,implying their high proliferous ability. Anti-BrdU and anti-S-100 staining showed numerous double staining positive SCs on the fascia: nucleus was stained deep blue while cytoplasm was stained red.ConclusionMany SCs with high proliferous ability were seen on the fascia, which can be used to repair transected nerve.
出处
《中国康复理论与实践》
CSCD
2004年第11期660-661,F003,共3页
Chinese Journal of Rehabilitation Theory and Practice
