摘要
目的 探讨骨髓间充质干细胞 (marrow mesenchmal stem cells,MSCs)移植至肌肉组织后的原位成肌分化情况。 方法 以 Bal B/ C雌性小鼠 36只 ,建立放射损伤合并创伤 (切割伤 +冻伤 )的严重肌损伤模型 ,再将分离扩增的雄性小鼠的单纯 MSCs及经 10μmol/ L 5 -氮杂胞苷 (5 - azacytidine,5 - Aza- CR)诱导 2 4 h的 MSCs以局部注射移植法 ,植入雌性小鼠正常肌肉组织和创伤后的肌肉组织 ,采用荧光标记的原位杂交法、免疫组织化学法在移植后 1、3、6、9、12及 15 d检测植入的 MSCs在肌肉组织原位的数量变化及成肌分化情况。 结果 植入的 MSCs数量随着时相延长而减少 ;单纯 MSCs植入正常肌肉组织 15 d,5 - Aza- CR诱导后的 MSCs植入正常肌肉组织 6 d,可见 MSCs分化为肌细胞 ,表达 desmin阳性 ;5 - Aza- CR诱导的 MSCs植入损伤肌肉组织 3d,单纯 MSCs植入损伤肌肉组织 6 d,可见 MSCs分化为肌细胞 ,表达 desmin阳性。 结论 MSCs局部移植至肌肉组织局部可实现成肌分化 ,但经 5 - Aza- CR诱导后的MSCs植入损伤肌肉组织的 MSCs其成肌分化时相明显早于单纯 MSCs植入正常肌组织的
Objective To investigate the myogenic differentiation of mesenchymal stem cells (MSCs) after being transplanted into the local muscle tissues. Methods The serious muscle injured model was established by the way of radiation injury, incising, and freezing injury in 36 mouses. Purified MSCs derived from bone marrow of male mouse and MSCs induced by 5 azacytidine(5 Aza CR) were transplanted into the local of normal muscle tissues and injured muscle tissues of femal mouse. The quantity of MSCs and the myogenic differentiation of implanted MSCs were detected by the method of double labeling, which included fluorescence in situ DNA hybridization (FISH) and immuno histochemistry on the 1st, 3rd, 6th, 9th, 12th, and 15th day after transplantation. Results The quantity of implanted MSCs decreased as time passed. MSCs' differentiation into myoblasts and positive expression of desmin were observed on the 15th day in purified MSCs group and on the 6th day in induced MSCs groups. Conclusion MSCs could differentiate into myoblasts after being implanted into the local of muscle tissues. The differentiation occurs earlier in the induced MSCs group than that in purified MSCs group.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2005年第1期70-73,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目 (30 2 0 0 1 4 2 )
国家重点基础研究发展规划资助项目 (973) :严重创伤早期全身性损害与组织修复的基础研究 (G1 9990 542 0 5)~~
