摘要
目的 :研究猪白细胞介素 2真核表达质粒 (pcDNA IL 2 )对猪繁殖与呼吸综合征病毒ORF5基因疫苗 (pcDNA PRRSV ORF5 )诱导BALB/c小鼠产生细胞免疫应答的影响。方法 :用pcDNA IL 2与PRRSVORF5基因疫苗 (pcDNA PRRSV ORF5 )共同免疫BALB/c小鼠 ,以空载质粒pcDNA为阴性对照 ,PBS为空白对照 ;采用流式细胞仪 (FACS)、淋巴细胞增殖试验(MTT法 )分别对小鼠外周血中CD4 + 、CD8+ T淋巴细胞数和淋巴细胞的转化功能进行了检测。结果 :pcDNA IL 2与pcDNA PRRSV ORF5共同免疫小鼠后外周血对ConA有明显的反应性 ,CD4 + 、CD8+ T淋巴细胞数在免疫后 7天显著超过对照组 ,共同组与单独pcDNA PRRSV ORF5组有显著差异 (P <0 0 1)。结论 :pcDNA PRRSV ORF5免疫小鼠能够诱导其机体产生良好的细胞免疫应答 ,猪pcDNA IL 2能够显著增强pcDNA PRRSV ORF5诱导小鼠细胞免疫的能力。
Objective:To study the effect of pig interleukin 2(IL 2) eukaryon expression plasmid on cellular immune responses of BALB/c mice immuned with pcDNA PRRSV ORF5 DNA vaccine.Methods:BALB/c mice were immunized with pcDNA PRRSV ORF5 DNA vaccine and pig interleukin 2(IL 2) eukaryon expression plasmid by the routes of co injection and DNA vaccine injection alone respectively, with PBS and pcDNA3 1(+) as controls. Fluoresecence Activated Cell Sorter(FACS),T lymphocyte proliferation test(MTT) were used to detect the number of CD4 +、CD8 + and the T lymphocyte proliferation in peripheral blood of mice vaccinated.Results:ConA response of T lymphocytes in blood was higher in experiment group than the control group ( P <0 01). The number of CD4 + and CD8 + T lymphocytes was significantly higher in experiment groups than that of the control group 7 days after vaccination. The experiment group vaccinated with DNA vaccine and pig IL 2 eukaryon expression plasmid co injection showed higher cellular immune response than that vaccinated with DNA vaccine alone.Conclusion:pcDNA PRRSV ORF5 DNA vaccine could induce higher response of cellular immune response and pig IL 2 eukaryon expression plasmid was effective adjuvant which could significantly enhance the immunogenicity of pcDNA PRRSV ORF5 DNA vaccine. [
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2005年第1期60-63,共4页
Chinese Journal of Immunology
基金
四川省生物技术项目 (0 1NG0 18 0 3 )
国家科技攻关重大项目(2 0 0 4BA90 1A0 3 )
四川省重点建设学科项目(SZD0 418)
关键词
猪白细胞介素2真核表达质粒
猪繁殖与呼吸综合征病毒ORF5基因疫苗
细胞免疫
小鼠
Pig interleukin 2(IL-2) eukaryon expression plasmid
Porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine
Cellular immune response
Mice
