摘要
目的 建立中药材天南星中夏佛托苷和异夏佛托苷的高效液相含量测定方法。方法 Waters分析柱XterraRp1 8(4 . 6mm× 2 5 0mm ,5 μm) ,流动相为乙腈 0 . 0 2 5mol·L-1 磷酸 (三乙胺调pH 3 . 5 ) (1 2 :88) ;柱温 30℃ ;流速 0 9mL·min-1 ;检测波长为2 70nm。结果 夏佛托苷线性范围为 0 . 0 5 31～ 0 . 6 90 6 μg ,回收率为 98 . 9%,RSD为 2 . 6 %;异夏佛托苷线性范围为 0 . 0 76 3～0 . 2 5 4 4 μg,回收率为 99 . 1 %,RSD为 2 . 0 %。结论 本方法快速、简便、准确、重现性较好 ,结果可靠 ,可为天南星药材的质量评价提供有效手段。
OBJECTIVE To establish an accurate HPLC method for the determination of schaftoside and isoschaftoside in Rhizoma Arisaematis (Tian-nanxing). METHODS Analytes were seperated at 30℃ using a waters Xeterra Rp18(4.6 mm×250 mm,5 μm) colum.The mobile phase consisted of acetonitrile-0.025 mol·L -1 phosphoric acid (12:88) with the flow rate of 0.9 mL·min -1. The detection wavelength was at 270 nm.RESULTS Within the range of 0.053 1～0.690 6 μg,schaftoside had good linearity. The average recovery was 98.9% (RSD=1.80%,n=5). The linear range of isoschaftoside was 0.076 3～0.254 4 μg.The average recovery was 99.1% (RSD=2.0%,n=5).CONCLUSION The method was simple and accurate.It will be used for the quality control of Rhizoma Arisaematis.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2005年第1期21-23,共3页
Chinese Pharmaceutical Journal
基金
"九五"国家重点科技攻关计划资助项目子专题 (96 90 3 0 2 0 2 )
