摘要
目的 探讨珍珠层聚乳酸 (nacre/ polylactic acid,N/ P)人工骨与同种异体成骨细胞复合 ,植入体内后异位成骨的作用机制 ,以及作为骨组织工程支架材料的可行性。 方法 成年雄性新西兰大白兔 18只 ,按 2、4和 8周3个时间点随机分成 3组 ,每组 6只。将体外培养的同种异体新西兰大白兔成骨细胞 ,种植到 N/ P人工骨材料上 ,并植入每只兔左侧背部皮下为实验组 ;以不复合成骨细胞的 N/ P人工骨材料植入右侧背部皮下作对照 ,分别于植入后不同时间点取材 ,经大体观察、组织学检查和免疫组织化学方法检测。 结果 实验组 2周时材料周围有少许炎性细胞聚集 ,4周时有类骨质形成 ,8周时有成熟骨组织形成 ,其中可见骨髓腔 ;对照组 2、4周时仅见大量纤维组织长入材料内 ,8周时材料内纤维组织增多 ,但无成骨发生。 结论 N/ P人工骨可作为理想的骨组织工程支架材料。
Objective To study the mechanism of ectopic osteogenesis of nacre/Polylactic acid (N/P) artificial bone combined with allogenic osteoblasts, and to explore the possibility as a scaffold material of bone tissue engineering. a(c)Methods The allogenic osteoblasts seeded onto N/P artificial bone were co cultured in vivo 1 week. The N/P artificial bone with allogenic osteoblasts were implanted subcutaneously into the left back sites of the New Zealand white rabbits in the experimental group and the simple N/P artificial bone into the right ones in the control group. The complexes were harvested and examined by gross observation, histologic analysis and immunohistochemical investigation 2, 4 and 8 weeks after implantation respectively. Results In experimental group, the osteoid formed after 4 weeks, and the mature bone tissue with bone medullary cavities formed after 8 weeks; but in control group there was no new bone formation instead of abundant fibrous tissue after 4 weeks, and more fibrous tissue after 8 weeks. Conclusion N/P artificial bone can be used as an optical scaffold material of bone tissue engineering. ?3/4
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2005年第2期114-117,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目 (30 2 71 32 1 )~~
