摘要
目的探讨以环孢素A(CsA)预处理供肾对肾脏损伤的防护作用。方法将30只日本大耳兔随机分为3组,每组10只,分别以生理盐水(对照组)、高渗枸橼酸盐嘌呤溶液(HCA液,灌注液对照组)及含CsA(30mg/L)的HCA液(实验组)原位灌洗左肾,然后采用自制的原位低温保存装置,将左肾置于0～4℃的低温水囊中,原位低温保存。2h后切除右肾,开放左肾血流,测定循环恢复后6h和24h后的血肌酐及尿素氮,24h时切取左肾标本,以免疫组织化学法测定左肾组织中热休克蛋白70(HSP70)和核因子κB(NFκB)的表达,原位末端标记法测定细胞凋亡率。结果实验组HSP70的表达水平明显高于两个对照组(P<0.01,P<0.01),NFκB的表达水平及细胞凋亡率明显低于两个对照组(P<0.01,P<0.01);实验组两个时间点的血肌酐及尿素氮水平明显低于两个对照组(P<0.01,P<0.01)。结论以CsA预处理肾脏,对肾脏的缺血再灌注损伤具有保护作用。
Objective To investigate the protective mechanism of cyclosporine A to renal autografts in situ.Methods Thirty rabbits were divided into three groups at random (n=10 in each group), subjected to left renal in situ perfusion with normal saline (control group), HC-A solution (HC-A group) and HC-A solution containing CsA (30 ml/L, experimental group) respectively. By ~using self-made in situ cryopreservation device, the left kidney was cryopreserved in situ at 0-4 ℃ for 2 h. Then the right kidney was dissected and the left renal blood flow was opened. At 6th h and 24th h after reperfusion, blood BUN and Cr levels were determined. At 24th h, the left kidneys were ~dissected for the detection of the expression of HSP70 and NF-κB in left renal tissues. The apoptotic rate in left renal tissues was assayed by in situ end-labeling method. Results In the experimental group, the expression level of HSP70 was significantly higher, while the expression of NF-κB and ~apoptotic rate significantly lower than in the other two groups (all P<~0.01 ). The blood Cr and BUN levels at two time points in experimental group were significantly lower than in the two groups (~P <~0.01 ). ~Conclusion Cyclosporine A preconditioning to kidney could protect the kidney from ~ischemia-reperfusion ~injury.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第1期44-46,共3页
Chinese Journal of Organ Transplantation
