摘要
Rb基因是一种抗癌基因,其产物Rb蛋白与细胞转化、细胞周期调节及转录调控有密切关系。许多肿瘤如视网膜母细胞瘤、乳腺癌、小细胞肺癌、前列腺癌、膀胱癌、骨肉瘤、软组织肉瘤等,都与Rb基因的失活或缺失有关。胃癌是消化道常见多发肿瘤,研究表明,其发生发展与多种癌基因的活化、抗癌基因的异常有关。我们用Southern印迹杂交法,以Rb基因为探针,检测了九例胃癌患者的癌组织和一个胃癌细胞株,发现它们与正常人血相比Rb基因全部呈现异常。其中5例发生Rb基因部分缺失,3例及人胃腺癌细胞株BGC-823全部缺失。这表明在胃癌发生过程中Rb基因的丢失是一个重要环节。我们相信用更灵敏的多聚酶链式反应(PCR)技术检测胃脱落细胞,对患癌危险性的早期预报会有很大帮助。同对,本实验也对肿瘤的基因治疗研究提供了靶细胞。
Rb gene is a cancer suppressor gene. Its product, Rb protein has close relation with the regulation of cell transformation, cell cycle and transcription. A lot of carcinomas such as retionblastoma, brest cancer, small cell lung cancer, prostate cancer, bladder cancer, oesteosacoma, soft tissue sarcoma etc. are related to the deletion or inactivation of Rb gene. Stomach cancer is a very common carcinoma of the gastrointestinal system. Researchers have shown that the development of stomach cancer is due to the activation of many oncogenes and the inactivation of antioncogenes. By Southern Blot hybridization, we have tested cancer tissues of nine stomach cancer patients and one cell line BGC-823 with Rb gene probe, finding that the Rb gene of all the tissues and the cell line are abnormal as compared with that of normal human blood tissues. Among the cancer tissues of the nine patients, five have partial deletion of Rb gene, three as well as the cell line have complete deletion of Rb gene. This indicates that the loss of Rb gene is the key link of cancer development. We believe that, with the more sensitive method of Polymerase Chain Reaction (PCR), we can test the fallen cells of stomach hoping that it would be helpful to the early diagnosis of stomach cancer. Meanwhile, this experiment provides target cells of gene therapy research of tumors.
基金
"八五"攻关
自然科学基金
关键词
RB基因
胃肿瘤
抗癌基因
Human Stomach Tissue
Rb gene
Deletion
Southern Blot Hybridization
